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Product Info Summary
| SKU: | A03076-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TRAF5 Antibody Picoband®
SKU/Catalog Number
A03076-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRAF5 Antibody Picoband® catalog # A03076-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAF5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03076-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TRAF5 recombinant protein (Position: K277-L557). Human TRAF5 shares 82.3% amino acid (aa) sequence identity with mouse TRAF5.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03076-1 is reactive to TRAF5 in Human, Mouse, Rat
Observed Molecular Weight
64 kDa
Calculated molecular weight
64.4 kDa
Background of TRAF5
TNF receptor-associated factor 5, also known as RNF84, is a protein that in humans is encoded by the TRAF5 gene. The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family. Fluorescence in situ hybridization confirmed the regional localization of human TRAF5 to chromosome 1q32.2-q32.3. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. It is an adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. It seems to be involved in apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03076-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat ovary tissue, mouse testis tissue, mouse thymus tissue, human MCF-7 whole cell, human A549 whole cell, human Hela whole cell
Validation Images & Assay Conditions
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Western blot analysis of TRAF5 using anti-TRAF5 antibody (A03076-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat ovary tissue lysate,
Lane 2: mouse testis tissue lysate,
Lane 3: mouse thymus tissue lysate,
Lane 4: human MCF-7 whole cell lysate,
Lane 5: human A549 whole cell lysate,
Lane 6: human Hela whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF5 antigen affinity purified polyclonal antibody (Catalog # A03076-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF5 at approximately 64KD. The expected band size for TRAF5 is at 64KD.
Specific Publications For Anti-TRAF5 Antibody Picoband® (A03076-1)
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