Mouse SEMA3F/Semaphorin-3F ELISA Kit PicoKine™

Mouse SEMA3F ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse SEMA3F in cell culture supernatants, serum and plasma (heparin, EDTA or citrate) . Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1734
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA or citrate)

Product Name

Mouse SEMA3F/Semaphorin-3F ELISA Kit PicoKine™

See all Semaphorin 3F products

SKU/Catalog Number

EK1734

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse SEMA3F ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse SEMA3F in cell culture supernatants, serum and plasma (heparin, EDTA or citrate) . Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse SEMA3F/Semaphorin-3F ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1734)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A19-T785

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1734 is reactive to Sema3f in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA or citrate)

Application Guarantee

EK1734 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1734-CAP Anti-Mouse Sema3f Pre-coated 96-well strip microplate 1
EK1734-ST Mouse Sema3f Standard 2 vials, 10 ng/tube
EK1734-DA Mouse Sema3f Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0060.0790.1170.2140.4030.7011.2182.037

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Semaphorin 3F, Dilution ratio of 1:1, concentration in serum is around 600pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse SEMA3F/Semaphorin-3F ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5923189661245907
Standard deviation3.4810.1656.454.0314.2168.03
CV (%)5.9%4.4%6.3%6.6%5.8%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Semaphorin 3F in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 159706460634.326.8%
Sample 223122422526123515.106.4%
Sample 389685692891389826.892.9%
*number of samples for each test n=16.

Gene/Protein Information For Sema3f (Source: Uniprot.Org, NCBI)

Uniprot ID

O88632

Gene ID

20350

Gene Name

Sema3f

Full Name

Semaphorin-3F

Weight

88.549kDa

Superfamily

semaphorin family

Alternative Names

sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F; sema III/F; sema IV; Sema3F; SEMA4; SEMA-IV; SEMAK; Semaphorin 3F; semaphorin III/F; semaphorin IV Sema3f|Sem, Sema4, Semak|sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F|semaphorin-3F|semaphorin IV

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Sema3f, check out the Sema3f Infographic

Sema3f infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Sema3f: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Mouse SEMA3F/Semaphorin-3F ELISA Kit PicoKine™

Question

Q: Can Semaphorin 3F ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

L. Smith

Verified customer

Asked: 2019-08-23

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-08-23

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-06-04

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Semaphorin 3F using the EK1734 Mouse Semaphorin 3F Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-05-15

Question

Q: What is the optimal O.D. value for Semaphorin 3F ELISA kit? I used your Semaphorin 3F ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Semaphorin 3F even though the O.D. values are not very high?

K. Patel

Verified customer

Asked: 2019-02-07

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Semaphorin 3F ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Semaphorin 3F that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-02-07

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

B. Collins

Verified customer

Asked: 2018-06-14

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-06-14

Question

Q: how to analyze ELISA data? I have obtained Semaphorin 3F level in plasma.

Verified Customer

Verified customer

Asked: 2017-08-11

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-08-11

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2015-03-14

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2015-03-14

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