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Product Info Summary
| SKU: | EK1554 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Syndecan-1/SDC1 ELISA Kit PicoKine®
View all Syndecan-1/CD138 ELISA kits
SKU/Catalog Number
EK1554
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Syndecan-1/SDC1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Sdc1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Syndecan-1/SDC1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1554)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q18-E252
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1554 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1554 is reactive to SDC1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1554 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Syndecan-1/CD138
Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1554-CAP | Anti-Mouse SDC1 Pre-coated 96-well strip microplate | 1 |
| EK1554-ST | Mouse SDC1 Standard | 2 vials, 10 ng/tube |
| EK1554-DA | Mouse SDC1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.081 | 0.163 | 0.210 | 0.317 | 0.416 | 0.700 | 1.163 | 1.928 |
Data Example Images
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Mouse Syndecan-1/SDC1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Syndecan-1/CD138, Dilution ratio of 1:1, concentration in serum and plasma is around 2 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Syndecan-1/SDC1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 326 | 1681 | 5402 | 348 | 1654 | 5887 |
| Standard deviation | 28.10 | 122.71 | 383.54 | 29.92 | 129.01 | 476.84 |
| CV (%) | 7.7% | 7.3% | 7.1% | 8.6% | 7.8% | 8.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Syndecan-1/CD138 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 326 | 374 | 349 | 330 | 344 | 18.99 | 5.5% |
| Sample 2 | 1681 | 1814 | 1841 | 1691 | 1756 | 71.47 | 4% |
| Sample 3 | 5402 | 5521 | 5466 | 5324 | 5428 | 73.45 | 1.3% |
Protein Target Info & Infographic
Gene/Protein Information For SDC1 (Source: Uniprot.Org, NCBI)
Gene Name
SDC1
Full Name
Syndecan-1
Weight
32.462kDa
Superfamily
syndecan proteoglycan family
Alternative Names
CD138 antigen; CD138; SDC; SDC1; SYND1heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; syndecan proteoglycan 1; syndecan; Syndecan1; Syndecan-1 SDC1 CD138, SDC, SYND1, syndecan syndecan 1 syndecan-1|CD138 antigen|heparan sulfate proteoglycan fibroblast growth factor receptor|syndecan proteoglycan 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SDC1, check out the SDC1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SDC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Syndecan-1/SDC1 ELISA Kit PicoKine® (EK1554)
Hello CJ!
EK1554 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Piperazine ferulate prevents high‑glucose‑induced filtration barrier injury of glomerular endothelial cells
Deficiency of AMPKα1 Exacerbates Intestinal Injury and Remote Acute Lung Injury in Mesenteric Ischemia and Reperfusion in Mice
Timmen,Melanie & Hidding,Heriburg & Götte,Martin & Khassawna,Thaqif & Kronenberg,Daniel & Stange,Richard. (2019). The Heparan Sulfate Proteoglycan Syndecan-1 Influences Local Bone Cell Communication via the RANKL/OPG Axis.10.1101/852590.
Species: Mouse
EK1554 usage in article: APP:ELISA, SAMPLE:MOUSE SERUM, DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
9 Customer Q&As for Mouse Syndecan-1/SDC1 ELISA Kit PicoKine®
Question
What is the recommended dilution ratio for mouse plasma using EK1554?
Verified customer
Asked: 2020-09-10
Answer
For the Mouse Syndecan-1/SDC1 ELISA Kit PicoKine™ (EK1554), 1:2 dilution is okay.
Boster Scientific Support
Answered: 2020-09-12
Question
Q: how do I analyze ELISA data? I have performed CD138 level in cell culture supernates .
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-11-21
Question
Q: if the enzyme conjugated CD138 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD138 antigen?
N. Miller
Verified customer
Asked: 2019-05-08
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-05-08
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-01-09
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-01-09
Question
Q: can I use citrate plasma as samples in Mouse CD138 Picokine® ELISA Kit (Catalog # EK1554)?
Verified Customer
Verified customer
Asked: 2018-12-29
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CD138 causing disruption of its protein structure. CD138 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD138 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-12-29
Question
Q: Can CD138 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
C. Collins
Verified customer
Asked: 2018-07-10
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-07-10
Question
Q: What is the optimal O.D. value for CD138 ELISA kit? I used your CD138 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD138 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2017-06-09
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD138 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD138 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2017-06-09
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CD138 using the EK1554 Mouse CD138 Picokine® ELISA Kit?
K. Adams
Verified customer
Asked: 2016-12-22
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2016-12-22
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2015-10-12
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2015-10-12
