Mouse TGF-Beta 3 ELISA Kit PicoKine™

Mouse TGF-Beta 3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tgfb3 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1104
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (EDTA) and urine.

Product Name

Mouse TGF-Beta 3 ELISA Kit PicoKine™

See all TGF-beta 3 products

SKU/Catalog Number

EK1104

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TGF-Beta 3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tgfb3 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse TGF-Beta 3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1104)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A301-S412

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1104 is reactive to Tgfb3 in Mouse samples

Validated Sample Types

cell culture supernatants, serum, plasma (EDTA) and urine.

Application Guarantee

EK1104 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1104-CAP Anti-Mouse Tgfb3 Pre-coated 96-well strip microplate 1
EK1104-ST Mouse Tgfb3 Standard 2 vials, 10 ng/tube
EK1104-DA Mouse Tgfb3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0250.0650.1050.1750.3110.6571.0541.877

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TGF-beta 3, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TGF-Beta 3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)58278101363286988
Standard deviation2.6115.0145.583.5915.7057.3
CV (%)4.5%5.4%4.5%5.7%5.7%5.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TGF-beta 3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 158645853583.896.7%
Sample 227830126630028614.875.1%
Sample 3101398295092896832.183.3%
*number of samples for each test n=16.

Gene/Protein Information For Tgfb3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P17125

Gene ID

21809

Gene Name

Tgfb3

Full Name

Transforming growth factor beta-3 proprotein

Weight

46.885kDa

Superfamily

TGF-beta family

Alternative Names

ARVD; ARVD1; FLJ16571; LDS5; RNHF; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor, beta 3 Tgfb3|TGF-beta-3, Tgfb-3|transforming growth factor, beta 3|transforming growth factor beta-3 proprotein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tgfb3, check out the Tgfb3 Infographic

Tgfb3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tgfb3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1104

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7 Customer Q&As for Mouse TGF-Beta 3 ELISA Kit PicoKine™

Question

Q: What is the optimal O.D. value for TGF-beta 3 ELISA kit? I used your TGF-beta 3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TGF-beta 3 even though the O.D. values are not very high?

K. Babu

Verified customer

Asked: 2020-05-27

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TGF-beta 3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TGF-beta 3 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-05-27

Question

Q: Can TGF-beta 3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-11-23

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-11-23

Question

Will product EK1104 work with mouse tissue lysates?

Verified customer

Asked: 2019-10-10

Answer

We haven't tested the Mouse TGF-Beta 3 ELISA Kit PicoKine EK1104 with mouse tissue lysates, so we cannot guarantee that it would work. Our suggestion for tissue homogenates is that endogenous biotin should be considered. Endogenous biotin in tissue homogenates might introduce false positive signal. Please run the test as described below to confirm if there is Endogenous biotin in the tissue lysate samples using the ELISA kit. • Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. • If no signal is produced, then you can work on the tissue sample by using the kit.

Boster Scientific Support

Answered: 2019-10-10

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-08-13

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

R. Parker

Verified customer

Asked: 2019-02-04

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-02-04

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-01-26

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-01-26

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification TGF-beta 3 using the EK1104 Mouse TGF-beta 3 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-06-13

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2016-06-13

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