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Product Info Summary
| SKU: | EK0517 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Thrombopoietin/TPO ELISA Kit PicoKine®
View all Thrombopoietin/THPO ELISA kits
SKU/Catalog Number
EK0517
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Thrombopoietin/TPO ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Thpo in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 30pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Thrombopoietin/TPO ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0517)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S22-T356
Sensitivity
<30 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0517 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0517 is reactive to THPO in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0517 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Thrombopoietin/THPO
Thrombopoietin (TPO) is also called megakaryocyte growth and development factor (MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0517-CAP | Anti-Mouse THPO Pre-coated 96-well strip microplate | 1 |
| EK0517-ST | Mouse THPO Standard | 2 vials, 10 ng/tube |
| EK0517-DA | Mouse THPO Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.055 | 0.098 | 0.124 | 0.215 | 0.399 | 0.768 | 1.468 | 2.036 |
Data Example Images
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Mouse TPO PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Thrombopoietin/THPO, Dilution ratio of 1:10, concentration in serum and plasma is around 6 ng/ml..
Some articles we found to cite concentrations of Thrombopoietin/THPO in samples: 12578637 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Thrombopoietin/TPO ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 92 | 307 | 851 | 85 | 330 | 918 |
| Standard deviation | 6.53 | 20.87 | 47.65 | 6.8 | 27.39 | 55.08 |
| CV (%) | 7.1% | 6.8% | 5.6% | 8% | 8.3% | 6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Thrombopoietin/THPO in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 92 | 87 | 100 | 93 | 93 | 4.63 | 4.9% |
| Sample 2 | 307 | 267 | 309 | 301 | 296 | 17 | 5.7% |
| Sample 3 | 851 | 915 | 849 | 807 | 855 | 38.58 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For THPO (Source: Uniprot.Org, NCBI)
Gene Name
THPO
Full Name
Thrombopoietin
Weight
37.823kDa
Superfamily
EPO/TPO family
Alternative Names
Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MKCSF; MK-CSF; ML; MPL ligand; MPLLG; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THCYT1; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF THPO MGDF, MKCSF, ML, MPLLG, THCYT1, TPO thrombopoietin thrombopoietin|MPL ligand|c-mpl ligand|megakaryocyte colony-stimulating factor|megakaryocyte growth and development factor|megakaryocyte stimulating factor|myeloproliferative leukemia virus oncogene ligand|prepro-thrombopoietin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on THPO, check out the THPO Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for THPO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Thrombopoietin/TPO ELISA Kit PicoKine® (EK0517)
Hello CJ!
EK0517 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Deficiency of platelet adhesion molecule CD226 causes megakaryocyte development and platelet hyperactivity
Improvement of icaritin on hematopoietic function in cyclophosphamide-induced myelosuppression mice
Extracted Triterpenes from Antrodia cinnamomea Reduce the Inflammation to Promote the Wound Healing via the STZ Inducing Hyperglycemia-Diabetes Mice Model
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Mouse Thrombopoietin/TPO ELISA Kit PicoKine®
Question
Q: how do I analyze ELISA data? I have performed Thrombopoietin level in plasma.
Verified Customer
Verified customer
Asked: 2020-08-17
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-08-17
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
T. Clark
Verified customer
Asked: 2020-03-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-03-08
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-04-30
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Thrombopoietin using the EK0517 Mouse Thrombopoietin Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-12-07
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2017-12-07
Question
Q: Can Thrombopoietin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
W. Patel
Verified customer
Asked: 2017-10-07
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2017-10-07
Question
Q: how can I thaw whole blood sample for Thrombopoietin ELISA after freezing?
W. Li
Verified customer
Asked: 2016-07-06
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Thrombopoietin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-07-06
