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Product Info Summary
| SKU: | A02133 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-EAAT1/SLC1A3 Picoband® Antibody
SKU/Catalog Number
A02133
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EAAT1/SLC1A3 Picoband® Antibody catalog # A02133. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EAAT1/SLC1A3 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02133)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human EAAT1/SLC1A3 recombinant protein (Position: Q34-D506).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02133 is reactive to SLC1A3 in Human, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
59.6 kDa
Background of SLC1A3
Excitatory amino acid transporter 1 (EAAT1) is a protein that, in humans, is encoded by the SLC1A3 gene. It is mapped to 5p13.2. This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02133 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue
FCM: U937 cell
Validation Images & Assay Conditions
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Western blot analysis of SLC1A3 using anti-SLC1A3 antibody (A02133).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC1A3 antigen affinity purified polyclonal antibody (Catalog # A02133) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC1A3 at approximately 60KD. The expected band size for SLC1A3 is at 60KD.
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Flow Cytometry analysis of U937 cells using anti-SLC1A3 antibody (A02133).
Overlay histogram showing U937 cells stained with A02133 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC1A3 Antibody (A02133, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-EAAT1/SLC1A3 Picoband® Antibody (A02133)
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Customer Q&As
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1 Customer Q&As for Anti-EAAT1/SLC1A3 Picoband® Antibody
Question
Please let me know whether this antibody binds to intra- or extra-cellular domains of EAAT1?
Verified customer
Asked: 2022-07-19
Answer
Thank you for your question. The immunogen sequence for A02133 is Q34-D506. https://www.uniprot.org/uniprotkb/P43003/entry
Boster Scientific Support
Answered: 2022-07-19
