Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine™

Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tlr2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1130
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine™

See all Tlr2 products

SKU/Catalog Number

EK1130

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tlr2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1130)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q25-L590

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1130 is reactive to Tlr2 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1130 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1130-CAP Anti-Mouse Tlr2 Pre-coated 96-well strip microplate 1
EK1130-ST Mouse Tlr2 Standard 2 vials, 10 ng/tube
EK1130-DA Mouse Tlr2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0160.0780.1280.2000.4120.8531.4342.097

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3041448466130114234925
Standard deviation21.5865.76195.7626.4891.07280.72
CV (%)7.1%4.5%4.2%8.8%6.4%5.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Tlr2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 13043233103073117.242.3%
Sample 21448137914871331141160.354.2%
Sample 346615150452247974782233.394.8%
*number of samples for each test n=16.

Gene/Protein Information For Tlr2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9QUN7

Gene ID

24088

Gene Name

Tlr2

Full Name

Toll-like receptor 2

Weight

89.449kDa

Superfamily

Toll-like receptor family

Alternative Names

CD282 antigen; CD282; TIL4CD282; TLR2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2 Tlr2|Ly10, Ly105|toll-like receptor 2|toll-like receptor 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tlr2, check out the Tlr2 Infographic

Tlr2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tlr2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1130

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

9 Customer Q&As for Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine™

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-07-29

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-07-29

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis TLR2 using the EK1130 Mouse TLR2 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2019-12-03

Question

What antibody the mouse is producing would be detected by your Mouse TLR2 ELISA Kit PicoKine after injecting the vaccine into a mouse that was made in CHO cells?

Verified customer

Asked: 2019-11-26

Answer

Unfortunately, Mouse TLR2 ELISA Kit PicoKine is only able to detect mouse TLR2 protein. It could not detect anti-TLR2 antibody.

Boster Scientific Support

Answered: 2019-11-26

Question

Q: is it okay to use citrate plasma as samples in Mouse TLR2 Picokine® ELISA Kit (Catalog # EK1130)?

Verified Customer

Verified customer

Asked: 2019-05-13

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TLR2 causing disruption of its protein structure. TLR2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TLR2 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-05-13

Question

Q: how to proceed with the analysis of ELISA data? I have obtained TLR2 level in serum.

Verified Customer

Verified customer

Asked: 2019-03-25

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-03-25

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-10-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-10-28

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Z. Anderson

Verified customer

Asked: 2017-06-18

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2017-06-18

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

H. Singh

Verified customer

Asked: 2014-07-07

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2014-07-07

Question

We have received a question regarding EK1130 and I am emailing to see if you can help. Here is the customer

Verified Customer

Verified customer

Asked: 2013-03-14

Answer

EK1130 is only able to detect mouse TLR2 protein. It could not detect anti-TLR2 antibody.

Boster Scientific Support

Answered: 2013-03-14

$369

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK1130
$369.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.