Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine®

Tlr2 ELISA kit for Mouse

Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tlr2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1130
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

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Product Name

Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine®

View all Tlr2 ELISA kits

SKU/Catalog Number

EK1130

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tlr2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1130)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q25-L590

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1130 is reactive to Tlr2 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1130 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Tlr2

Toll-like receptor 2 also known as TLR-2 is a protein that in humans is encoded by the TLR2 gene. TLR2 has also been designated as CD282 (cluster of differentiation 282). TLR-2 plays a role in the immune system. TLR-2 is a membrane protein, a receptor, which is expressed on the surface of certain cells and recognizes foreign substances and passes on appropriate signals to the cells of the immune system. TLR2 expresses as 4- and 4.4-kb mRNAs in heart, brain, and muscle.

Kit Components

Catalog Number Description Quantity
EK1130-CAP Anti-Mouse Tlr2 Pre-coated 96-well strip microplate 1
EK1130-ST Mouse Tlr2 Standard 2 vials, 10 ng/tube
EK1130-DA Mouse Tlr2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0160.0780.1280.2000.4120.8531.4342.097

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3041448466130114234925
Standard deviation21.5865.76195.7626.4891.07280.72
CV (%)7.1%4.5%4.2%8.8%6.4%5.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Tlr2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 13043233103073117.242.3%
Sample 21448137914871331141160.354.2%
Sample 346615150452247974782233.394.8%
*number of samples for each test n=16.

Gene/Protein Information For Tlr2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9QUN7

Gene ID

24088

Gene Name

Tlr2

Full Name

Toll-like receptor 2

Weight

89.449kDa

Superfamily

Toll-like receptor family

Alternative Names

CD282 antigen; CD282; TIL4CD282; TLR2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2 Tlr2|Ly10, Ly105|toll-like receptor 2|toll-like receptor 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tlr2, check out the Tlr2 Infographic

Tlr2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tlr2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1130 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

HuR induces inflammatory responses in HUVECs and murine sepsis via binding to HMGB1

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9 Customer Q&As for Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine®

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-07-29

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-07-29

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis TLR2 using the EK1130 Mouse TLR2 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2019-12-03

Question

What antibody the mouse is producing would be detected by your Mouse TLR2 ELISA Kit PicoKine after injecting the vaccine into a mouse that was made in CHO cells?

Verified customer

Asked: 2019-11-26

Answer

Unfortunately, Mouse TLR2 ELISA Kit PicoKine is only able to detect mouse TLR2 protein. It could not detect anti-TLR2 antibody.

Boster Scientific Support

Answered: 2019-11-26

Question

Q: is it okay to use citrate plasma as samples in Mouse TLR2 Picokine® ELISA Kit (Catalog # EK1130)?

Verified Customer

Verified customer

Asked: 2019-05-13

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TLR2 causing disruption of its protein structure. TLR2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TLR2 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-05-13

Question

Q: how to proceed with the analysis of ELISA data? I have obtained TLR2 level in serum.

Verified Customer

Verified customer

Asked: 2019-03-25

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-03-25

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-10-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-10-28

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Z. Anderson

Verified customer

Asked: 2017-06-18

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2017-06-18

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

H. Singh

Verified customer

Asked: 2014-07-07

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2014-07-07

Question

We have received a question regarding EK1130 and I am emailing to see if you can help. Here is the customer

Verified Customer

Verified customer

Asked: 2013-03-14

Answer

EK1130 is only able to detect mouse TLR2 protein. It could not detect anti-TLR2 antibody.

Boster Scientific Support

Answered: 2013-03-14

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