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Product Info Summary
| SKU: | EK1227 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse TNFRSF14/HVEM ELISA Kit PicoKine®
View all HVEM/TNFRSF14 ELISA kits
SKU/Catalog Number
EK1227
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse TNFRSF14/HVEM ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TNFRSF14/HVEM ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1227)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: Q29-V207
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1227 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1227 is reactive to TNFRSF14 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1227 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HVEM/TNFRSF14
Tumor necrosis factor receptor superfamily member 14 (TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a bidirectional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1227-CAP | Anti-Mouse TNFRSF14 Pre-coated 96-well strip microplate | 1 |
| EK1227-ST | Mouse TNFRSF14 Standard | 2 vials, 10 ng/tube |
| EK1227-DA | Mouse TNFRSF14 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.004 | 0.069 | 0.135 | 0.230 | 0.495 | 0.905 | 1.521 | 2.050 |
Data Example Images
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Mouse TNFRSF14/HVEM PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect HVEM/TNFRSF14, Dilution ratio of 1:1, concentration in serum and plasma is 300-500 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TNFRSF14/HVEM ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 29 | 218 | 439 | 28 | 197 | 475 |
| Standard deviation | 1.59 | 14.38 | 26.77 | 1.56 | 16.15 | 31.82 |
| CV (%) | 5.5% | 6.6% | 6.1% | 5.6% | 8.2% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of HVEM/TNFRSF14 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 29 | 31 | 29 | 29 | 29 | 0.86 | 2.9% |
| Sample 2 | 218 | 201 | 191 | 189 | 199 | 11.47 | 5.7% |
| Sample 3 | 439 | 461 | 413 | 382 | 423 | 29.49 | 6.9% |
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF14 (Source: Uniprot.Org, NCBI)
Gene Name
TNFRSF14
Full Name
Tumor necrosis factor receptor superfamily member 14
Weight
30.392kDa
Superfamily
tumor necrosis factor receptor superfamily
Alternative Names
ATAR; CD270 antigen; CD270; CD40-like protein; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; HVEM; HVEMTR2HVEAATAR; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator); Tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2 TNFRSF14 ATAR, CD270, HVEA, HVEM, LIGHTR, TR2 TNF receptor superfamily member 14 tumor necrosis factor receptor superfamily member 14|CD40-like protein|herpes virus entry mediator A|tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)|tumor necrosis factor receptor-like gene2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFRSF14, check out the TNFRSF14 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse TNFRSF14/HVEM ELISA Kit PicoKine® (EK1227)
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No publications found for EK1227
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Mouse TNFRSF14/HVEM ELISA Kit PicoKine®
Question
Q: Can HVEM ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-08-19
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-08-19
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis HVEM using the EK1227 Mouse HVEM Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2020-02-14
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
H. Anand
Verified customer
Asked: 2019-12-29
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-12-29
Question
Q: how can I thaw whole blood sample for HVEM ELISA after freezing?
K. Green
Verified customer
Asked: 2019-05-24
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test HVEM for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-05-24
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-10-29
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-10-29
Question
Q: is it okay to use citrate plasma as samples in Mouse HVEM Picokine® ELISA Kit (Catalog # EK1227)?
W. Babu
Verified customer
Asked: 2018-10-15
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of HVEM causing disruption of its protein structure. HVEM may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the HVEM ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-10-15
Question
Q: if the enzyme conjugated HVEM antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the HVEM antigen?
Verified Customer
Verified customer
Asked: 2018-02-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-02-11
