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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and serum.|
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Mouse VEGFR2/KDR ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse VEGFR2/KDR ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Kdr in cell culture supernatants and serum. Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse VEGFR2/KDR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0590)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A20-E762
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0590 is reactive to Kdr in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
EK0590 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of VEGFR2/KDR/Flk-1
Vascular endothelial growth factor receptor 2 (VEGFR-2) is a VEGF receptor. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It is mapped to 4q12. This gene encodes one of the two receptors of the VEGF. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells. In addition to those, VEGFR-2 is also a positive functional marker defining stem cells and distinguishing them from progenitors.
|EK0590-CAP||Anti-Mouse Kdr Pre-coated 96-well strip microplate||1|
|EK0590-ST||Mouse Kdr Standard||2 vials, 10 ng/tube|
|EK0590-DA||Mouse Kdr Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse VEGFR2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect VEGFR2/KDR/Flk-1, Dilution ratio of 1:20, concentration in serum is 20-40ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse VEGFR2/KDR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of VEGFR2/KDR/Flk-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Kdr (Source: Uniprot.Org, NCBI)
Vascular endothelial growth factor receptor 2
protein kinase superfamily
CD309 antigen; CD309; EC 2.7.10; EC 220.127.116.11; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2 Kdr|6130401C07, Flk, Flk-, Flk-1, Flk1, Krd-1, Ly73, VEGF, VEGFR, VEGFR-2, VEGFR2, orv, sVEGFR-2|kinase insert domain protein receptor|vascular endothelial growth factor receptor 2|VEGF receptor-2|fetal liver kinase 1|kinase NYK|protein-tyrosine kinase receptor flk-1|soluble vascular endothelial growth factor receptor 2|vascular endothelial growth factor receptor-3*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Kdr, check out the Kdr Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Kdr: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0590 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
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