Multiplex ELISA Kit For Human Angiogenesis (9-plex)

ELISA kit for Multiplex detection

The Multiplex Human Angiogenesis (9-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of nine biomarkers or analytes: Ang-2, FGF, HGF, IL-8, PDGF-BB, TIMP-1, TIMP-2, TNFα, VEGF. Attention: This product requires a special plate reader. Please make sure you have the proper equipment before ordering. For details, please contact [email protected].

Product Info Summary

Species: Human
Sample Type: Serum, EDTA/Heparin Plasma
Volume Required per Well: Min 25 µl
Assay Length: 2.25 hours
Specificity: All analytes < 1% intra-panel cross-reactivity
Assay Type: Planar Based Multiplexed ELISA
Detection Method: Chemiluminescent
Multiplex Format: 96-well solid plate
Catalog: MEK1021
Manufacturer Reference Number: 150233HU
Analytes:Ang-2, FGF, HGF, IL-8, PDGF-BB, TIMP-1, TIMP-2, TNFα, VEGF

Product Overview

The Multiplex Human Angiogenesis (9-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of nine biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all nine markers in the panel within 2.25 hours.

The Multiplex Human Angiogenesis (9-plex) provides researchers an easy to use and cost effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing nine distinct capture antibodies in a defined array to the bottom of each well. Our high quality reagents help ensure the accuracy of your results.

Assay Ranges

AnalyteAssay TypeCalibrator RangeUpper Limit of Quantification (ULOQ)Lower Limit of Quantification (LLOQ)Limit of Detection
Ang-2Sandwich10,000 – 13.72 (pg/mL)7000 (pg/mL)17.8 (pg/mL)3.90 (pg/mL)
FGFSandwich5000 – 6.86 (pg/mL)3500 (pg/mL)8.9 (pg/mL)1.27 (pg/mL)
HGFSandwich 14000 – 19.20 (pg/mL)9800 (pg/mL)25 (pg/mL)7.20 (pg/mL)
IL-8Sandwich2000 – 2.74 (pg/mL)1400 (pg/mL)3.6 (pg/mL)2.70 (pg/mL)
PDGFSandwich4000 – 5.49 (pg/mL)2800 (pg/mL)7.1 (pg/mL)2.20 (pg/mL)
TIMP-1Sandwich12697 – 17.42 (pg/mL)8888 (pg/mL)22.6 (pg/mL)12.95 (pg/mL)
TIMP2Sandwich20000 – 27.43 (pg/mL)14000 (pg/mL)35.7 (pg/mL)15.90 (pg/mL)
TNF-αSandwich6500 – 8.92 (pg/mL)4522 (pg/mL)11.5 (pg/mL)7.74 (pg/mL)
VEGFSandwich2000 – 2.74 (pg/mL)1400 (pg/mL)3.6 (pg/mL)2.70 (pg/mL)

Kit Components

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.

Reagents Include:

Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips

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SKU:MEK1021

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The multiplex ELISA kits require special plate readers. For more info see special plate reader. The $800 demo option is for new customer only, see more info at demo options.
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