Multiplex ELISA Kit For Human Cytokine HS Screen (15-Plex)

The Multiplex Human Cytokine HS Screen (15-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 15 biomarkers or analytes. IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10 IL-12p70, IL-13, IL-15, IL-17, IL-23, IFNγ, TNFα, TNFβ

Product Info Summary

Species: Human
Sample Type: Serum, EDTA Plasma
Volume Required per Well: Min 25 µl
Assay Length: 4.83 hours
Specificity: All analytes < 1% intra-panel cross-reactivity
Assay Type: Planar Based Multiplexed ELISA
Detection Method: Chemiluminescent
Multiplex Format: 96-well solid plate
Catalog: MEK1007
Manufacturer Reference Number: 112433HU
Analytes:IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10 IL-12p70, IL-13, IL-15, IL-17, IL-23, IFNγ, TNFα, TNFβ

Product Overview

The Multiplex Human Cytokine HS Screen (15-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 15 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 15 markers in the panel within 4.83 hours. The Multiplex Human Cytokine HS Screen (15-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 15 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Assay Ranges

Units Calibrator Range LLD Limit
IL-1α pg/ml 1300-1.78 1.40
IL-1β pg/ml 3070-4.21 1.75
IL-2 pg/ml 50-0.07 0.07
IL-4 pg/ml 23-0.03 0.02
IL-5 pg/ml 360-0.49 0.20
IL-6 pg/ml 350-0.48 0.40
IL-10 pg/ml 950-1.30 1.10
IL-12p70 pg/ml 3000-4.12 3.00
IL-13 pg/ml 340-0.47 0.23
IL-15 pg/ml 950-1.30 0.43
IL-17 pg/ml 1200-1.65 1.48
IL-23 pg/ml 9000-12.35 1.50
IFNγ pg/ml 80-0.11 0.10
TNFα pg/ml 440-0.60 0.31
TNFβ pg/ml 470-0.64 0.57

Kit Components

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.

Reagents Include:

Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (20): Adhesive strips

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MEK1007
The multiplex ELISA kits require special plate readers. For more info see special plate reader. The $800 demo option is for new customer only, see more info at demo options.
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