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Product Info Summary
| SKU | MEK2004 |
| Species | Human |
| Sample Type | Serum, EDTA/Heparin Plasma |
| Volume Required per Well | Min 25 µl |
| Assay Length | 2.25 hours |
| Specificity | All analytes < 1% intra-panel cross-reactivity |
| Assay Type | Planar Based Multiplexed ELISA |
| Detection Method | Chemiluminescent |
| Multiplex Format | 96-well solid plate |
| Manufacturer Reference Number: | 112033HU |
|---|---|
| Analytes | IL-1α, IL-4, IL-6, TNFα |
Product info
Product Overview
The Multiplex ELISA Kit For Human Cytokine Panel 2 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-1α, IL-4, IL-6, TNFα).
Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 2.25 hours. Multiplex ELISA Kit For Human Cytokine Panel 2 (4-Plex) provides scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.
Assay Ranges
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| IL-1α | Sandwich | 4,000 -5.49 (pg/mL) | 2,800 (pg/mL) | 7.1 (pg/mL) | 5.43 (pg/mL) | 6% | 7% | 86% |
| IL-4 | Sandwich | 1,676 – 2.30 (pg/mL) | 1,173 (pg/mL) | 3.0 (pg/mL) | 1.79 (pg/mL) | 6% | 6% | 94% |
| IL-6 | Sandwich | 3,000 – 4.12 (pg/mL) | 2,100 (pg/mL) | 5.3 (pg/mL) | 3.47 (pg/mL) | 7% | 4% | 104% |
| TNFα | Sandwich | 6,500 – 8.92 (pg/mL) | 4,522 (pg/mL) | 11.5 (pg/mL) | 7.74 (pg/mL) | 6% | 5% | 98% |
Kit Components
Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Substrate A: Liquid, 3mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips
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