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Product Info Summary
| Catalog: | MEK1005 |
|---|---|
| Species: | Human |
| Sample Type: | Serum, EDTA/Heparin Plasma |
| Volume Required per Well: | Min 25 µl |
| Assay Length: | 2.25 hours |
| Specificity: | All analytes < 1% intra-panel cross-reactivity |
| Assay Type: | Planar Based Multiplexed ELISA |
| Detection Method: | Chemiluminescent |
| Multiplex Format: | 96-well solid plate |
| Manufacturer Reference Number: | 110933HU |
| Analytes: | IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-23, TNFα, TNFβ |
Product info
Product Overview
The Multiplex Human Cytokine – Screen (16-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Multiple analyte measurements are achieved by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well of a 96-well plate. Our high-quality reagents help ensure the accuracy and precision of your results.
Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.25 hours. The Multiplex Human Cytokine – Screen (16-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample.
Assay Ranges
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| IFNγ | Sandwich | 8,000 - 10.97 (pg/mL) | 5,600 (pg/mL) | 14.3 (pg/mL) | 5.49 (pg/mL) | 8% | 4% | 97% |
| IL-1α | Sandwich | 4,000 - 5.49 (pg/mL) | 2,800 (pg/mL) | 7.1 (pg/mL) | 2.74 (pg/mL) | 6% | 7% | 86% |
| IL-1β | Sandwich | 12,226 - 16.77 (pg/mL) | 8,558 (pg/mL) | 21.8 (pg/mL) | 8.39 (pg/mL) | 8% | 5% | 94% |
| IL-2 | Sandwich | 5,213 – 7.15 (pg/mL) | 3,649 (pg/mL) | 9.3 (pg/mL) | 3.58 (pg/mL) | 6% | 4% | 99% |
| IL-4 | Sandwich | 1,676 – 2.30 (pg/mL) | 1,173 (pg/mL) | 3.0 (pg/mL) | 1.15 (pg/mL) | 6% | 6% | 94% |
| IL-5 | Sandwich | 3,000 – 4.12 (pg/mL) | 2,100 (pg/mL) | 5.3 (pg/mL) | 2.06 (pg/mL) | 10% | 7% | 95% |
| IL-6 | Sandwich | 3,000 – 4.12 (pg/mL) | 2,100 (pg/mL) | 5.3 (pg/mL) | 2.06 (pg/mL) | 7% | 4% | 104% |
| IL-8 | Sandwich | 2,000 – 2.74 (pg/mL) | 1,400 (pg/mL) | 3.6 (pg/mL) | 1.37 (pg/mL) | 12% | 7% | 100% |
| IL-10 | Sandwich | 8,039 – 11.03 (pg/mL) | 5,627 (pg/mL) | 14.3 (pg/mL) | 5.51 (pg/mL) | 7% | 5% | 103% |
| IL-12p70 | Sandwich | 3,000 – 4.12 (pg/mL) | 2,100 (pg/mL) | 5.3 (pg/mL) | 2.06 (pg/mL) | 7% | 7% | 103% |
| IL-13 | Sandwich | 3,000 – 4.12 (pg/mL) | 2,100 (pg/mL) | 5.3 (pg/mL) | 2.06 (pg/mL) | 6% | 7% | 98% |
| IL-15 | Sandwich | 10,733 – 14.72 (pg/mL) | 7,513 (pg/mL) | 19.1 (pg/mL) | 7.36 (pg/mL) | 10% | 7% | 96% |
| IL-17A | Sandwich | 16,772 – 23.01 (pg/mL) | 11,740 (pg/mL) | 29.9 (pg/mL) | 11.50 (pg/mL) | 7% | 7% | 95% |
| IL-23 | Sandwich | 41700 – 57.20 (pg/mL) | 21,000 (pg/mL) | 120 (pg/mL) | 28.60 (pg/mL) | 10% | 5% | 105% |
| TNFα | Sandwich | 6,500 – 8.92 (pg/mL) | 4,522 (pg/mL) | 11.5 (pg/mL) | 4.46 (pg/mL) | 6% | 5% | 98% |
| TNFβ | Sandwich | 4,000 – 5.49 (pg/mL) | 2,800 (pg/mL) | 7.1 (pg/mL) | 2.74 (pg/mL) | 8% | 5% | 96% |
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips
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2 Customer Q&As for Multiplex ELISA Kit For Human Cytokine - Screen (16-plex)
Question
Please let me know how many separate samples we may test?
Verified customer
Asked: 2022-01-03
Answer
This human kit is in a 96-well plate. 16 of the wells are reserved for the calibration curve leaving 80 wells open for samples. Depending on the testing protocol, up to 80 samples may be tested. We do recommend running samples in triplicate, or at least in duplicate, and many customers also have controls they plan to run. This means that while a customer can run 80 samples per plate, most test between 25 and 40.
Boster Scientific Support
Answered: 2022-01-03
Question
Does the Multiplex ELISA Kit For Human Cytokine - Screen (16-Plex) (MEK1005) only work with the Q-plex reader?
Verified customer
Asked: 2021-11-02
Answer
If your reader is not Q-plex, please check if it meets the following requirements. Here are the minimum qualifications that the imager must meet to be able to image our plates: 1. The imager has a light‐tight imaging chamber, a non‐illumination or chemi-luminescent mode, and can export grayscale 16‐bit TIFF files. 2. Camera‐based imagers must also have at least 1 MP resolution (4+ MP, flat‐field correction, and a cooled sensor is preferred). 3. In addition, the device must also be able to export grayscale 16-bit TIFF files. We can not guarantee that our multiplex kit will work on your imager if it is not a Q-plex reader.
Boster Scientific Support
Answered: 2021-11-03
