Multiplex ELISA Kit For Human Cytokine - Stripwells (16-plex)

The Multiplex Human Cytokine – Stripwells (16-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10 IL-12p70, IL-13, IL-15, IL-17, IL-23, IFNγ, TNFα, TNFβ

Product Info Summary

Species: Human
Sample Type: Serum, Plasma, and Urine
Volume Required per Well: Min 25 µl
Assay Length: 2.25 hours
Specificity: All analytes < 1% intra-panel cross-reactivity
Assay Type: Planar Based Multiplexed ELISA
Detection Method: Chemiluminescent
Multiplex Format: 96-well solid plate
Catalog: MEK1001
Manufacturer Reference Number: 110333HU
Analytes:IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10 IL-12p70, IL-13, IL-15, IL-17, IL-23, IFNγ, TNFα, TNFβ

Product Overview

The Multiplex Human Cytokine – Stripwells (16-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.25 hours. The Multiplex Human Cytokine HS Screen (15-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Assay Ranges

Units Calibrator Range LLD Limit
IL-1α pg/ml 4000 – 5.49 5.43
IL-1β pg/ml 10000 - 13.72 10.14
IL-2 pg/ml 4000 – 5.49 3.16
IL-4 pg/ml 2000 – 2.74 2.14
IL-5 pg/ml 3000 – 4.12 2.90
IL-6 pg/ml 3000 – 4.11 3.47
IL-8 pg/ml 2000 – 2.74 2.70
IL-10 pg/ml 3000 – 4.11 4.10
IL-12p70 pg/ml 3000 – 4.12 3.20
IL-13 pg/ml 3000 – 4.12 2.86
IL-15 pg/ml 3000 – 4.12 1.92
IL-17 pg/ml 4000 - 5.49 5.40
IL-23 pg/ml 30000 - 41.15 25.00
IFNγ pg/ml 8000 - 10.97 4.95
TNFα pg/ml 4000 - 5.49 5.20
TNFβ pg/ml 4000 - 5.49 5.29

Kit Components

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.

Reagents Include:

Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (20): Adhesive strips

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5 Customer Q&As for Multiplex ELISA Kit For Human Cytokine - Stripwells (16-plex)

Question

Is it possible to use cell culture supernatants as the sample type for MEK1001?

Verified customer

Asked: 2020-12-18

Answer

Yes, you can use cell culture supernatants on the Multiplex ELISA Kit For Human Cytokine - Stripwells (16-Plex) (MEK1001).

Boster Scientific Support

Answered: 2020-12-21

Question

What kind of plate reader does MEK1001 require?

Verified customer

Asked: 2020-12-18

Answer

The Multiplex ELISA Kit For Human Cytokine - Stripwells (16-Plex) (MEK1001) is built to be used with Q-View imagers.

Boster Scientific Support

Answered: 2020-12-21

Question

Can those buffers of MEK1001 use separation and do independent experiment different times?

Verified customer

Asked: 2020-06-20

Answer

Yes, there are three separate vials that the customer would reconstitute one at a time as they need to use them. There are enough reagents to run the Multiplex ELISA Kit For Human Cytokine - Stripwells (16-Plex) (MEK1001) kit three different times.

Boster Scientific Support

Answered: 2020-06-23

Question

What plate reader is needed for the MEK1001 kit? Is this compatible with BioTek Synergy HT?

Verified customer

Asked: 2019-12-18

Answer

We do not support third party imagers and since there are so many models to keep track of, we are not certain which ones are compatible with our kits. The following are the requirements for a third party imager to be compatible with our plates: -The imager should have a light‐tight imaging chamber, a non‐illumination or chemi-luminescent mode, and can export grayscale 16‐bit TIFF files. -Camera‐based imagers must also have at least 1 MP resolution (4+ MP, flat‐field correction, and a cooled sensor is preferred).

Boster Scientific Support

Answered: 2019-12-23

Question

Does the MEK1001 kit come with any analysis software or is it not needed?

Verified customer

Asked: 2019-12-18

Answer

Unforunately not. The software must be purchased for $1,980 for long-term use (license does not expire). A free 60 day trial can be downloaded once you purchase the product.

Boster Scientific Support

Answered: 2019-12-23

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MEK1001
The multiplex ELISA kits require special plate readers. For more info see special plate reader. The $800 demo option is for new customer only, see more info at demo options.
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