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Product Info Summary
|Sample Type:||Heparin Plasma|
|Volume Required per Well:||Min 5 µl|
|Assay Length:||3.33 hours|
|Specificity:||All analytes < 1% intra-panel cross-reactivity|
|Assay Type:||Planar Based Multiplexed ELISA|
|Multiplex Format:||96-well solid plate|
|Manufacturer Reference Number:||110449RT|
|Analytes:||AGP, CRP, Ferritin, sTfR, RBP4, Tg, HRP2|
The Multiplex Human Micronutrient Array (7-plex) is a fully quantitative chemiluminescent assay allowing concurrent measurement of biomarkers used in nutritional assessment in heparinized plasma samples. Analytes CRP, Ferritin, Thyroglobulin (Tg), and HRP2 are sandwich ELISAs while AGP, sTfR and RBP4 are competitive ELISAs. This product is alternatively known in previous publications as Multiplex Micronutrient Assessment Tool (MMAT).
A Human Micronutrient Assay kit contains a 96-well plate, with each well featuring the 7-plex along with a positive control and all the other reagents necessary to perform the test. Our high quality reagents help ensure the accuracy of your results.
Using just 5 µl of plasma (with 45 µl of diluent) per well, up to 80 wells can be tested for all seven markers in the panel in 3.33 hours. Multiplex Arrays provide scientists with an easy-to-use and cost effective means of generating a biomarker profile for each sample.
|Units||Calibrator Range||LLD Limit|
|AGP||g/L||0.36 - 0.0005||0.0019|
|CRP||mg/L||5.61 - 0.0077||0.006|
|Ferritin||μg/L||115 - 0.1578||0.2057|
|sTfR||mg/L||107 - 0.1468||0.1917|
|RBP4||μmol/L||1.01 - 0.0014||0.0054|
|Tg||μg/L||13.7 - 0.0188||0.0244|
|HRP2||μg/L||0.96 - 0.0013||0.0017|
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (20): Adhesive strips
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