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Product Info Summary
| Catalog: | MEK1004 |
|---|---|
| Species: | Rat |
| Sample Type: | Cell Culture Supernatant, Serum, Plasma |
| Volume Required per Well: | Min 25 µl |
| Assay Length: | 3 hours |
| Specificity: | All analytes < 1% intra-panel cross-reactivity |
| Assay Type: | Planar Based Multiplexed ELISA |
| Detection Method: | Chemiluminescent |
| Multiplex Format: | 96-well solid plate |
| Manufacturer Reference Number: | 111649RT |
| Analytes: | IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, TNFα |
Product info
Product Overview
The Multiplex Rat Cytokine – Inflammation (9-Plex) is a fully quantitative ELISA-based Chemiluminescent assay allowing the concurrent measurement of nine biomarkers or analytes. Using just 25 µl of sample per well, up to 80 samples can be assayed for all nine markers in the panel within 3 hours.
The Multiplex Rat Cytokine – Inflammation (9-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing nine distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Assay Ranges
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| IFNγ | Sandwich | 6,000 - 8.23 (pg/mL) | 4,200 (pg/mL) | 10.7 (pg/mL) | 4.12 (pg/mL) | 9.80% | 9.70% | 97% |
| IL-1α | Sandwich | 5,400 - 7.41 (pg/mL) | 3,780 (pg/mL) | 9.6 (pg/mL) | 3.7 (pg/mL) | 8.50% | 8.80% | 101% |
| IL-1β | Sandwich | 3,200 - 4.39 (pg/mL) | 2,240 (pg/mL) | 5.7 (pg/mL) | 2.19 (pg/mL) | 7.70% | 7.90% | 102% |
| IL-2 | Sandwich | 9,800 - 13.44 (pg/mL) | 6,860 (pg/mL) | 17.5 (pg/mL) | 6.72 (pg/mL) | 9.90% | 10.10% | 99% |
| IL-4 | Sandwich | 4,000 - 5.49 (pg/mL) | 2,800 (pg/mL) | 7.1 (pg/mL) | 2.74 (pg/mL) | 9.40% | 4.30% | 97% |
| IL-6 | Sandwich | 83,000 - 114 (pg/mL) | 58,100 (pg/mL) | 148 (pg/mL) | 56.93 (pg/mL) | 8.00% | 9.10% | 104% |
| IL-10 | Sandwich | 14,000 - 19.20 (pg/mL) | 9,800 (pg/mL) | 25 (pg/mL) | 9.6 (pg/mL) | 10.80% | 8.40% | 101% |
| IL-12p70 | Sandwich | 92,000 - 126 (pg/mL) | 64,400 (pg/mL) | 164.1 (pg/mL) | 63.1 (pg/mL) | 10.50% | 7.10% | 101% |
| TNFα | Sandwich | 37,000 - 50.75 (pg/mL) | 25,900 (pg/mL) | 66 (pg/mL) | 25.38 (pg/mL) | 9.40% | 8.40% | 116% |
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips
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2 Customer Q&As for Multiplex ELISA Kit For Rat Cytokine - Inflammation (9-plex)
Question
Is it possible to use MEK1004 with brain tissue or milk samples?
Verified customer
Asked: 2019-11-14
Answer
The Multiplex ELISA Kit For Rat Cytokine - Inflammation (9-Plex) (MEK1004) should be compatible with brain tissue. The issue would be how the samples are prepared. It depends on what kind of sample the customer is looking to test (brain cross section pieces, chunks, just brain cells, ect.). The cells should be lysated or homogenized for the best results. Milk samples are not validated for our kits as we have not tested them.
Boster Scientific Support
Answered: 2019-11-18
Question
Which reader is compatible with MEK1004? Do you know if Chemidoc can be optimized for this use?
Verified customer
Asked: 2019-10-16
Answer
The only imager that has been guaranteed to work with our ELISA kit is the Q-view imager LS. We are not sure how to optimize the Multiplex ELISA Kit For Rat Cytokine - Inflammation (9-Plex) (MEK1004) with Chemidoc.
Boster Scientific Support
Answered: 2019-10-16
