Multiplex ELISA Kit For Rat Cytokine - Inflammation (9-plex)

The Multiplex Rat Cytokine – Inflammation (9-Plex) is a fully quantitative ELISA-based Chemiluminescent assay allowing the concurrent measurement of nine biomarkers or analytes. IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, TNFα

Product Info Summary

Species: Rat
Sample Type: Serum, EDTA Plasma
Volume Required per Well: Min 25 µl
Assay Length: 2.75 hours
Specificity: All analytes < 1% intra-panel cross-reactivity
Assay Type: Planar Based Multiplexed ELISA
Detection Method: Chemiluminescent
Multiplex Format: 96-well solid plate
Catalog: MEK1004
Manufacturer Reference Number: 110449RT
Analytes:IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, TNFα

Product Overview

The Multiplex Rat Cytokine – Inflammation (9-Plex) is a fully quantitative ELISA-based Chemiluminescent assay allowing the concurrent measurement of nine biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all nine markers in the panel within 2.75 hours. The Multiplex Rat Cytokine – Inflammation (9-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing nine distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Assay Ranges

Units Calibrator Range LLD Limit
IFNγ pg/ml 17343 - 23.79 18.40
IL-1α pg/ml 17705 - 24.29 17.50
IL-1β pg/ml 19221 - 26.37 15.00
IL-2 pg/ml 81609 - 111.95 89.70
IL-4 pg/ml 7055 - 9.68 3.18
IL-6 pg/ml 136761 - 187.60 94.70
IL-10 pg/ml 7814 - 10.72 5.21
IL-12p70 pg/ml 50464 - 69.22 45.10
TNFα pg/ml 28218 - 38.71 22.60

Kit Components

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.

Reagents Include:

Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (20): Adhesive strips

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2 Customer Q&As for Multiplex ELISA Kit For Rat Cytokine - Inflammation (9-plex)

Question

Is it possible to use MEK1004 with brain tissue or milk samples?

Verified customer

Asked: 2019-11-14

Answer

The Multiplex ELISA Kit For Rat Cytokine - Inflammation (9-plex) should be compatible with brain tissue. The issue would be how the samples are prepared. It depends on what kind of sample the customer is looking to test (brain cross section pieces, chunks, just brain cells, ect.). The cells should be lysated or homogenized for the best results. Milk samples are not validated for our kits as we have not tested them.

Boster Scientific Support

Answered: 2019-11-18

Question

Which reader is compatible with the Multiplex ELISA Kit For Rat Cytokine - Inflammation (9-plex) MEK1004? Do you know if chemidoc can be optimize for this use?

Verified customer

Asked: 2019-10-16

Answer

The only imager that has been guaranteed to work with our ELISA kit is the Q-view imager LS. We are not sure how to optimize this kit with Chemidoc.

Boster Scientific Support

Answered: 2019-10-16

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MEK1004
The multiplex ELISA kits require special plate readers. For more info see special plate reader. The $800 demo option is for new customer only, see more info at demo options.
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