Western Blot

Western Blot WB Technical Blogs

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Protein Analysis Kits and Reagents Fall Catalog

September 22, 2017

Keywords: time-saving kits and reagents, western blot reagents catalog prices, extraction and isolation kits, low price Coomassie blue, BCA and Micro BCA kits, back-to-school promotion

Save time this year with our sensitive all-in-one kits and ready-to-use reagents available now. BosterBio's Membrane Protein Extraction Kit, Mitochondira Isolation Kit, Cytoplasmic and Nuclear Protein Extraction Kit, and more are convenient research tools for protein analysis. Get the WB reagents catalog with troubleshooting eBook included! Full protocols included in our downloadable Western Blot eBook. Restock your labs before back-to-school sale ends in October, all BosterBio products are performance guaranteed or your money back! Fast global delivery and re-ordering options, at a comparitively low price. See details here...

Western Blot Tips and Tricks: Blocking Optimization

August 25, 2017

Keywords: Western Blot Blocking Optimization, wb membrane blocking troubleshooting, optimizing blocking and washing step of WB experiments

Do you Western Blot? Utilize our summary workflow for a standard Western Blot protocol, with necessary reagents for each step included! Following protein transfer, it is important to block the unreacted sites on the membrane using inert proteins and/or nonionic detergent to reduce levels of nonspecific protein binding during the assay. Blocking buffers should block all unreacted sites without disrupting target protein-membrane interactions or affect epitope availability. In this newsletter, learn the 3 key factors to consider when choosing an appropriate blocking agent for your specific Western Blot experiment!

3 Guidelines for Using Secondary Antibodies

Apr 16, 2017

Keywords: secondary antibody, technical tips, troubleshooting, Flow cytometry antibodies

The purpose of using a secondary antibody is to amplify the primary antibody and antigen interactions signals. If you need secondary antibodies for your experiment, then you probably know there are many elements to consider when choosing the right antibody such as species reactivity. Dr. Booster has a few guidelines for you to keep in mind about secondary antibodies. Be sure to take a look and confirm everything is good to go.

Troubleshooting Guides

Troubleshooting Guides

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Troubleshooting Guides