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Western Blot
Western Blot WB Technical Blogs
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Protein Analysis Kits and Reagents Fall Catalog
September 22, 2017
Keywords: time-saving kits and reagents, western blot reagents catalog prices, extraction and isolation kits, low price Coomassie blue, BCA and Micro BCA kits, back-to-school promotion
Save time this year with our sensitive all-in-one kits and ready-to-use reagents available now. BosterBio's Membrane Protein Extraction Kit, Mitochondira Isolation Kit, Cytoplasmic and Nuclear Protein Extraction Kit, and more are convenient research tools for protein analysis. Get the WB reagents catalog with troubleshooting eBook included! Full protocols included in our downloadable Western Blot eBook. Restock your labs before back-to-school sale ends in October, all BosterBio products are performance guaranteed or your money back! Fast global delivery and re-ordering options, at a comparitively low price. See details here...
Western Blot Tips and Tricks: Blocking Optimization
August 25, 2017
Keywords: Western Blot Blocking Optimization, wb membrane blocking troubleshooting, optimizing blocking and washing step of WB experiments
Do you Western Blot? Utilize our summary workflow for a standard Western Blot protocol, with necessary reagents for each step included! Following protein transfer, it is important to block the unreacted sites on the membrane using inert proteins and/or nonionic detergent to reduce levels of nonspecific protein binding during the assay. Blocking buffers should block all unreacted sites without disrupting target protein-membrane interactions or affect epitope availability. In this newsletter, learn the 3 key factors to consider when choosing an appropriate blocking agent for your specific Western Blot experiment!
3 Guidelines for Using Secondary Antibodies
Apr 16, 2017
Keywords: secondary antibody, technical tips, troubleshooting, Flow cytometry antibodies
The purpose of using a secondary antibody is to amplify the primary antibody and antigen interactions signals. If you need secondary antibodies for your experiment, then you probably know there are many elements to consider when choosing the right antibody such as species reactivity. Dr. Booster has a few guidelines for you to keep in mind about secondary antibodies. Be sure to take a look and confirm everything is good to go.
Troubleshooting Guides
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.
Troubleshooting GuidesRelated Pages
Boster Technical Blogs and News
CD40 can be found on several cell types, such as B cells, macrophages, dendritic cells, and fibroblasts, demonstrating its critical role in immune response and host defense. Furthermore, their research suggests that antibodies against SARS-CoV spike ...
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Troubleshooting guides IHC, WB, PCR Reagents & Kits Boster offers a full range of high quality buffers, reagents, and kits for everyday lab use. Our antibodies are validated using WB, IHC, and flow cytometry against a panel of over 250 tissues and un...
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Each kit contains a 96-well plate of the relevant biomarker panel and includes all required reagents to perform testing. Using just 50 µl of sample per well, up to 80 samples can be assayed for all markers in the panel within 2.5 hours. This promoti...
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These small proteins are produced by a broad range of cells, which include macrophages, B-Cells, T-Cells, basophils, mast cells, etc. On the surface of T cells are PD-1 (programmed cell death-1) receptors whose ligands include PD-L1 and PD-L2. When t...
See MoreOptimizing Western Blot Blocking
There are three factors to consider when choosing an appropriate blocking agent for your specific protocol: (Click the meme for blocking optimization tips!) We advise reviewing the data sheet for any recommended blocking agents because antibodies can...
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