Receive a free secondary antibody with your primary antibody purchase. Browse Secondary Antibodies
Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
Boster provides plate-based multiplex cytokine immunoassay service for analytes from human, mouse and rat. Contact us today and get a free consultation.
Boster offers custom recombinant protein expression service. Available expresssion systems include E. Coli, Yeast, Insect and Mamalian Cells. Get a free consultation today.
Boster offers custom DNA synthesis service for as low as $0.08 per bp. Any gene cloning into any vector, 100% accuracy, Fast turn around time. Get a free consultation today.
Boster offers a full range of lab services for immunohistochemistry (IHC) and immunofluorescence (IF). Get a free consultation today.
Want to have all technical references by your finger tips? Download our FREE eBooks for WB, IHC, ELISA, Flow cytometry and Molecular biology here. These ebooks contain detailed information regarding the principles, protocols troubleshooting tips and optimization tips for their respective assays. Handy for newbies and veterans alike.
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The purpose of using a secondary antibody is to amplify the primary antibody and antigen interactions. Before choosing a secondary antibody for your experiment, ask yourself the following 3 questions to help you choose the appropriate one:
What is the host species of the primary antibody?
The secondary antibody should be raised against the host in which the primary antibody was raised. For example, if the primary antibody was raised in rabbit, you will need an anti-rabbit secondary antibody raised in a species other than rabbit.
What is the isotype of the primary antibody?
The secondary antibody has to be directed specifically against the isotype of the primary antibody. For example, the polyclonal primary antibodies are generally of the IgG isotype and thus the corresponding (species specific) secondary antibody needs be an anti-IgG antibody. The monoclonal antibodies, which are commonly raised in mice and rabbits, have the IgG1 isotype and require the suitable secondary antibody.
Does the secondary antibody cross react with species other than that of the primary antibody?
There is always a chance that the secondary antibody might cross-react with the endogenous immunoglobulins or other proteins of the sample. Therefore, it is a good idea to go for secondary antibodies that have been pre-adsorbed with the serum of the same species as the sample.
In addition, the primary antibodies of a multicolour panel will likely have been raised in different hosts and thus the corresponding secondary antibodies might cross-react with more than one primary antibody. The solution for this is to utilize cross-adsorbed secondary antibodies which have been treated against sera of one or more species.
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