The purpose of using a secondary antibody is to amplify the primary antibody and antigen interactions. Before choosing a secondary antibody for your experiment, ask yourself the following 3 questions to help you choose the appropriate one:

  1. What is the host species of the primary antibody?

    The secondary antibody should be raised against the host in which the primary antibody was raised. For example, if the primary antibody was raised in rabbit, you will need an anti-rabbit secondary antibody raised in a species other than rabbit.

  2. What is the isotype of the primary antibody?

    The secondary antibody has to be directed specifically against the isotype of the primary antibody. For example, the polyclonal primary antibodies are generally of the IgG isotype and thus the corresponding (species specific) secondary antibody needs be an anti-IgG antibody. The monoclonal antibodies, which are commonly raised in mice and rabbits, have the IgG1 isotype and require the suitable secondary antibody.

  3. Does the secondary antibody cross react with species other than that of the primary antibody?

    There is always a chance that the secondary antibody might cross-react with the endogenous immunoglobulins or other proteins of the sample. Therefore, it is a good idea to go for secondary antibodies that have been pre-adsorbed with the serum of the same species as the sample.

    In addition, the primary antibodies of a multi-color panel will likely have been raised in different hosts and thus the corresponding secondary antibodies might cross-react with more than one primary antibody. The solution for this is to utilize cross-adsorbed secondary antibodies which have been treated against sera of one or more species.

Boster provides over 70 secondary antibodies with different conjugations for several applications. Browse our secondary antibodies here.

Unlock precision in research with our Antibody Pair Development Service – revolutionize your experiments today!

*Note to educators: You are permitted to share Boster Bio's resources and PDFs on your class websites and lab websites. Please make sure to cite or link to the origin.

Buy Primary Antibody, Get Secondary FREE

Buy Primary Antibody Get Secondary FREE

Boster Guarantee

All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet. We promise to thoroughly investigate any concerns about the quality of our products; if you encounter a problem with a Boster antibody or ELISA kit, our technical support team will respond with personalized advice within 24 hours. If we can’t make your experiment work, we will refund your purchase in full or provide a replacement free of charge.

100% Satisfaction Guaranteed

About Boster Biological Technology

Here are some quick facts about bosterbio.com

26
Years

2300+
Publications

4.8 / 5
Rating on biocompare.com

16000+
Antibodies

900+
ELISA Kits