PDCD4 Colorimetric Cell-Based ELISA Kit

Pdcd4 ELISA kit, cell-based

The PDCD4 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PDCD4 protein expression profile in cells. The kit can be used for measuring the relative amounts of PDCD4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PDCD4.

Product Info Summary

SKU: EKC1445
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

PDCD4 Colorimetric Cell-Based ELISA Kit

See all Pdcd4 products

SKU/Catalog Number

EKC1445

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The PDCD4 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PDCD4 protein expression profile in cells. The kit can be used for measuring the relative amounts of PDCD4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PDCD4.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

PDCD4 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1445)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1445 is reactive to Pdcd4 in Human, Mouse, Rat samples

Application Guarantee

EKC1445 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Pdcd4 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For Pdcd4 (Source: Uniprot.Org, NCBI)

Gene Name

Pdcd4

Full Name

Programmed cell death protein 4

Weight

51.702kDa

Superfamily

PDCD4 family

Alternative Names

H731; H731nuclear antigen H731; MGC33046; MGC33047; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; PDCD4; programmed cell death 4 (neoplastic transformation inhibitor); programmed cell death protein 4; Protein 197/15a Pdcd4|D19Ucl, D19Ucla1, MA-, Ma3, T, Tis|programmed cell death 4|programmed cell death protein 4|protein MA-3|topoisomerase-inhibitor suppressed protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Pdcd4, check out the Pdcd4 Infographic

Pdcd4 infographic

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In this infographic you will see the following information for Pdcd4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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