PEA-15 Colorimetric Cell-Based ELISA Kit

PEA-15 ELISA kit, cell-based

The PEA-15 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PEA-15 protein expression profile in cells. The kit can be used for measuring the relative amounts of PEA-15 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PEA-15.

Product Info Summary

SKU: EKC1450
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

PEA-15 Colorimetric Cell-Based ELISA Kit

See all PEA-15 products

SKU/Catalog Number

EKC1450

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The PEA-15 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PEA-15 protein expression profile in cells. The kit can be used for measuring the relative amounts of PEA-15 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PEA-15.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

PEA-15 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1450)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1450 is reactive to PEA15 in Human, Mouse, Rat samples

Application Guarantee

EKC1450 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-PEA-15 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For PEA15 (Source: Uniprot.Org, NCBI)

Gene Name

PEA15

Full Name

Astrocytic phosphoprotein PEA-15

Weight

15.04kDa

Alternative Names

HMAT1; HMAT115kD; HUMMAT1H; MAT1H; PEA15; PEA-15; PED; PED15 kDa phosphoprotein enriched in astrocytes; phosphoprotein enriched in astrocytes 15; Phosphoprotein enriched in diabetes PEA15 HMAT1, HUMMAT1H, MAT1, MAT1H, PEA-15, PED, PED-PEA15, PED/PEA15 proliferation and apoptosis adaptor protein 15 astrocytic phosphoprotein PEA-15|15 kDa phosphoprotein enriched in astrocytes|homolog of mouse MAT-1 oncogene|mammary transforming gene 1, mouse, homolog of|phosphoprotein enriched in astrocytes 15|phosphoprotein enriched in diabetes|proliferation and apoptosis adaptor 15

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PEA15, check out the PEA15 Infographic

PEA15 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PEA15: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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