PGE2 Competitive ELISA Kit PicoKine®

Product Name

PGE2 Competitive ELISA Kit PicoKine®

SKU/Catalog Number

EK6001

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

PGE2 Competitive ELISA Kit PicoKine® (96 Tests). Quantitate PGE2 in cell culture supernatants, serum and plasma (heparin, EDTA),urine. Sensitivity: 1.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

PGE2 Competitive ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK6001)

Clonality of Antibodies

See Datasheet for details

Sensitivity

<20 pg/ml

Assay Range

2pg/ml-20,000pg/ml

Standard Dilution Instructions

See datasheet of EK6001 for more details

Cross-reactivity

There is no detectable cross-reactivity with other cytokines.

Reactive Species

EK6001 is reactive to in samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA),urine.

Application Guarantee

EK6001 is guaranteed for ELISA in by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of

Prostaglandin E2 (PGE2), also known as dinoprostone, is a naturally occurring prostaglandin with oxytocic properties that is used as a medication.Dinoprostone is used in labor induction, bleeding after delivery, termination of pregnancy, and in newborn babies to keep the ductus arteriosus open.In babies it is used in those with congenital heart defects until surgery can be carried out. It is also used to manage gestational trophoblastic disease.It may be used within the vagina or by injection into a vein.

Kit Components

Catalog Number	Description	Quantity
EK6001-CAP	Anti- Pre-coated 96-well strip microplate	1
EK6001-ST	Standard	2 vials, 20 ng/tube
EK6001-DA	Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	2	20	200	2000	20000
O.D.	2.301	2	1.88	1.193	0.812	0.387

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of PGE2 Competitive ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis