Product Info Summary
SKU: | EKC1454 |
---|---|
Size: | 1 kit, containing one 96-well plate and all necessary reagents |
Reactive Species: | Human, Mouse, Rat |
Application: | ELISA |
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Product info
Product Name
PGP9.5 Colorimetric Cell-Based ELISA Kit
See all UCH-L1/PGP9.5 products
SKU/Catalog Number
EKC1454
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The PGP9.5 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PGP9.5 protein expression profile in cells. The kit can be used for measuring the relative amounts of PGP9.5 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PGP9.5.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
PGP9.5 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1454)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1454 is reactive to UCHL1 in Human, Mouse, Rat samples
Application Guarantee
EKC1454 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Reagent | Quantity | Container |
---|---|---|
96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
10x TBS | 24 ml (10x) | Clear |
Quenching Buffer | 24 ml (1x) | Clear |
Blocking Buffer | 50 ml (1x) | Clear |
15x Wash Buffer | 50 ml (15x) | Clear |
100x Anti-UCHL1 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
Primary Antibody Diluent | 12 ml (1x) | Clear |
Ready-to-Use Substrate | 12 ml (1x) | Brown |
Stop Solution | 12 ml (1x) | Clear |
Crystal Violet Solution | 6ml (1x) | Glass |
SDS Solution | 24 ml (1x) | Clear |
Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
Click image to see more details
Boster Kit Box
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Western blot analysis of extracts from 293 cells
Protein Target Info & Infographic
Gene/Protein Information For UCHL1 (Source: Uniprot.Org, NCBI)
Gene Name
UCHL1
Full Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Weight
24.824kDa
Superfamily
peptidase C12 family
Alternative Names
EC 3.4.19.12; EC 6.-; Neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP9.5Uch-L1; PGP95; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin C-terminal hydrolase; Ubiquitin thioesterase L1; UCHL1; UCH-L1 UCHL1 HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, Uch-L1 ubiquitin C-terminal hydrolase L1 ubiquitin carboxyl-terminal hydrolase isozyme L1|epididymis luminal protein 117|epididymis secretory protein Li 53|neuron cytoplasmic protein 9.5|ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)|ubiquitin thioesterase L1|ubiquitin thiolesterase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on UCHL1, check out the UCHL1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for UCHL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PGP9.5 Colorimetric Cell-Based ELISA Kit (EKC1454)
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