Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) Antibody

Boster Bio Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) Antibody catalog # M01018-5. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.

Product Info Summary

SKU: M01018-5
Size: 100ul
Reactive Species: Bovine, Equine, Human, Mouse, Pig, Rat
Host: Chicken
Application: IF, IHC, ICC, WB

Product Name

Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) Antibody

See all UCH-L1/PGP9.5 products

SKU/Catalog Number

M01018-5

Size

100ul

Description

Boster Bio Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) Antibody catalog # M01018-5. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01018-5)

Host

Chicken

Contents

Purified IgY preparation with 5mM NaN3 at 20-30 mg/mL

Clonality

Polyclonal

Immunogen

Recombinant full length human UCHL1 purified from E. coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01018-5 is reactive to UCHL1 in Bovine, Equine, Human, Mouse, Pig, Rat

Applications

M01018-5 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB: 1:10,000. IF/ICC and IHC: 1:1,000.

Validation Images & Assay Conditions

Gene/Protein Information For UCHL1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P09936

Gene ID

7345

Gene Name

UCHL1

Full Name

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Weight

24.824kDa

Superfamily

peptidase C12 family

Alternative Names

EC 3.4.19.12; EC 6.-; Neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP9.5Uch-L1; PGP95; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin C-terminal hydrolase; Ubiquitin thioesterase L1; UCHL1; UCH-L1 UCHL1 HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, Uch-L1 ubiquitin C-terminal hydrolase L1 ubiquitin carboxyl-terminal hydrolase isozyme L1|epididymis luminal protein 117|epididymis secretory protein Li 53|neuron cytoplasmic protein 9.5|ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)|ubiquitin thioesterase L1|ubiquitin thiolesterase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on UCHL1, check out the UCHL1 Infographic

UCHL1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for UCHL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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15 Customer Q&As for Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) Antibody

Question

We have observed staining in rat brain. Are there any suggestions? Is anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

From literature brain does express UCHL1. From Uniprot.org, UCHL1 is expressed in anterior cingulate cortex, lung muscle, brain, cajal-retzius cell fetal brain cortex, among other tissues. Regarding which tissues have UCHL1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 2947814
Lung, and Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-02-10

Question

We have tried in the past anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody for WB on cajal-retzius cell fetal brain cortex in the past. I am using equine, and We are going to use the antibody for IHC next. Our lab want to know about examining cajal-retzius cell fetal brain cortex as well as anterior cingulate cortex in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-11-20

Answer

I took a look at the website and datasheets of our anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody and it appears that M01018-5 has been validated on equine in both WB and IHC. Thus M01018-5 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in equine even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-20

Question

I see that the anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-02

Question

Do you have a BSA free version of anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 available?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-06-25

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for anterior cingulate cortex using anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-05-30

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-30

Question

Will anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 work for WB with anterior cingulate cortex?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

According to the expression profile of anterior cingulate cortex, UCHL1 is highly expressed in anterior cingulate cortex. So, it is likely that anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 will work for WB with anterior cingulate cortex.

Boster Scientific Support

Answered: 2019-05-28

Question

I am interested in to test anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 on mouse anterior cingulate cortex for research purposes, then I may be interested in using anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Johnson

Verified customer

Asked: 2019-02-13

Answer

The products we sell, including anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-02-13

Question

Is this M01018-5 anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody reactive to the isotypes of UCHL1?

Verified Customer

Verified customer

Asked: 2019-02-08

Answer

The immunogen of M01018-5 anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody is Recombinant full length human UCHL1 purified from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-02-08

Question

We were satisfied with the WB result of your anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody. However we have been able to see positive staining in lung muscle cytoplasm. using this antibody. Is that expected? Could you tell me where is UCHL1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-11-23

Answer

Based on literature, lung muscle does express UCHL1. Generally UCHL1 expresses in cytoplasm. Regarding which tissues have UCHL1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 2947814
Lung, and Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-11-23

Question

Does M01018-5 anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-10-25

Answer

You can see on the product datasheet, M01018-5 anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-10-25

Question

Please see the WB image, lot number and protocol we used for anterior cingulate cortex using anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5. Please let me know if you require anything else.

K. Edwards

Verified customer

Asked: 2018-07-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-09

Question

I was wanting to use your anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody for WB for mouse anterior cingulate cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse anterior cingulate cortex identification?

G. Wu

Verified customer

Asked: 2018-02-14

Answer

As indicated on the product datasheet, M01018-5 anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody has been tested for IHC, WB on bovine, equine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-02-14

Question

We are currently using anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says bovine, equine, human, mouse, pig, rat. Is it likely that the antibody can work on pig tissues as well?

C. Yang

Verified customer

Asked: 2015-12-08

Answer

The anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody (M01018-5) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-12-08

Question

Is a blocking peptide available for product anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody (M01018-5)?

G. Wu

Verified customer

Asked: 2014-12-08

Answer

We do provide the blocking peptide for product anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody (M01018-5). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-12-08

Question

Can you help my question with product M01018-5, anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Banerjee

Verified customer

Asked: 2013-02-05

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01018-5 anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-02-05

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