Pig porcine CXCL14 ELISA Kit PicoKine®

Product Name

Pig porcine CXCL14 ELISA Kit PicoKine®

SKU/Catalog Number

EK1285-PO

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Pig porcine CXCL14 ELISA Kit PicoKine® (96 Tests). Quantitate Pig CXCL14 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 60pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Pig porcine CXCL14 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1285-PO)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: S35-E111

Sensitivity

<60 pg/ml

Assay Range

125 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

See datasheet of EK1285-PO for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1285-PO is reactive to CXCL14 in Pig samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1285-PO is guaranteed for ELISA in Pig by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CXCL14

Chemokine (C-X-C motif) ligand 14 (CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK (for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.

Kit Components

Catalog Number	Description	Quantity
EK1285-PO-CAP	Anti-Pig CXCL14 Pre-coated 96-well strip microplate	1
EK1285-PO-ST	Pig CXCL14 Standard	2 vials, 10 ng/tube
EK1285-PO-DA	Pig CXCL14 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	125	250	500	1000	2000	4000
O.D.	0.052	0.126	0.187	0.313	0.573	1.118	1.929	nan

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Pig porcine CXCL14 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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