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Product Info Summary
|Description:||Transient overexpression lysate of phosphatidylinositol glycan anchor biosynthesis, class Z (PIGZ)|
PIGZ (NM_025163) Human Over-expression Lysate
Transient overexpression lysate of phosphatidylinositol glycan anchor biosynthesis, class Z (PIGZ)
Storage & Handling
Cite This Product
PIGZ (NM_025163) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080235)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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PIGZ Human Over-expression Lysates NM_025163.
Western blot validation of overexpression lysate (LS080235) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PIGZ (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for PIGZ (Source: Uniprot.org, NCBI)
GPI mannosyltransferase 4
glycosyltransferase 22 family
class Z; EC 2.4.1; EC 2.4.1.-; FLJ12768; GPI mannosyltransferase 4; GPI mannosyltransferase IV; GPI-MT-IV; hSMP3; phosphatidylinositol glycan anchor biosynthesis, class Z; Phosphatidylinositol-glycan biosynthesis class Z protein; PIG-Z; SMP3 mannosyltransferase; SMP3SMP3 homolog PIGZ GPI-MT-IV, PIG-Z, SMP3 phosphatidylinositol glycan anchor biosynthesis class Z GPI mannosyltransferase 4|GPI mannosyltransferase IV|SMP3 mannosyltransferase|dol-P-Man dependent GPI mannosyltransferase|phosphatidylinositol glycan, class Z|phosphatidylinositol-glycan biosynthesis class Z protein*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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