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Product Info Summary
| SKU: | LS000661 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of polymerase (RNA) III (DNA directed) polypeptide D, 44kDa (POLR3D) |
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Product info
Product Name
POLR3D (NM_001722) Human Over-expression Lysate
SKU/Catalog Number
LS000661
Size
100 µg
Description
Transient overexpression lysate of polymerase (RNA) III (DNA directed) polypeptide D, 44kDa (POLR3D)
Storage & Handling
Cite This Product
POLR3D (NM_001722) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000661)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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POLR3D Human Over-expression Lysates NM_001722.
Western blot validation of overexpression lysate (LS000661) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with POLR3D (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for POLR3D (Source: Uniprot.org, NCBI)
Gene Name
POLR3D
Full Name
DNA-directed RNA polymerase III subunit RPC4
Weight
44.396kDa
Superfamily
eukaryotic RPC4/POLR3D RNA polymerase subunit family
Alternative Names
BN51 (BHK21) temperature sensitivity complementing; BN51; BN51TDNA-directed RNA polymerase III 47 kDa polypeptide; DNA-directed RNA polymerase III subunit D; DNA-directed RNA polymerase III subunit RPC4; polymerase (RNA) III (DNA directed) polypeptide D, 44kDa; Protein BN51; RNA polymerase III 47 kDa subunit; RNA polymerase III subunit C4; RPC4; RPC53 homolog; temperature sensitive complementation, cell cycle specific, tsBN51; TSBN51RPC53 POLR3D BN51T, C53, RPC4, RPC53, TSBN51 RNA polymerase III subunit D DNA-directed RNA polymerase III subunit RPC4|BN51 (BHK21) temperature sensitivity complementing|DNA-directed RNA polymerase III 47 kDa polypeptide|DNA-directed RNA polymerase III subunit D|RNA polymerase III 47 kDa subunit|RNA polymerase III subunit C4|RPC53 homolog|polymerase (RNA) III (DNA directed) polypeptide D, 44kDa|polymerase (RNA) III subunit D|temperature sensitive complementation, cell cycle specific, tsBN51
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on POLR3D, check out the POLR3D Infographic
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In this infographic, you will see the following information for POLR3D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For POLR3D (NM_001722) Human Over-expression Lysate (LS000661)
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