Anti-BCAS4 Antibody

BASC4 antibody

Boster Bio Anti-BCAS4 Antibody (Catalog # A13963). Tested in WB, IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: A13963
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-BCAS4 Antibody

View all BASC4 Antibodies

SKU/Catalog Number

A13963

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-BCAS4 Antibody (Catalog # A13963). Tested in WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BCAS4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13963)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human BCAS4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A13963 is reactive to BCAS4 in Human

Applications

A13963 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

22.758kDa

Background of BASC4

A chromosomal aberration involving BCAS4 has been found in some breast carcinoma cell lines. Translocation t(17;20)(q23;q13) with BCAS3. Belongs to the cappuccino family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.

Baerlund M., Genes Chromosomes Cancer 35:311-317(2002).
Deloukas P., Nature 414:865-871(2001).
Ota T., Nat. Genet. 36:40-45(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For BCAS4 (Source: Uniprot.org, NCBI)

Gene Name

BCAS4

Full Name

Breast carcinoma-amplified sequence 4

Weight

22.758kDa

Superfamily

cappuccino family

Alternative Names

BCAS4/BCAS3 fusion; breast carcinoma amplified sequence 4; breast carcinoma amplified sequence 4/3 fusion protein; breast carcinoma-amplified sequence 4; CNOL; FLJ20495 BCAS4 CNOL breast carcinoma amplified sequence 4 breast carcinoma-amplified sequence 4|BCAS4/BCAS3 fusion|breast carcinoma amplified sequence 4/3 fusion protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BCAS4, check out the BCAS4 Infographic

BCAS4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BCAS4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Find answers in Q&As, reviews.

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7 Customer Q&As for Anti-BCAS4 Antibody

Question

Is this A13963 anti-BCAS4 antibody reactive to the isotypes of BCAS4?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

The immunogen of A13963 anti-BCAS4 antibody is Synthesized peptide derived from internal of human BCAS4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-18

Question

Please see the WB image, lot number and protocol we used for lymph using anti-BCAS4 antibody A13963. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-29

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-29

Question

We are currently using anti-BCAS4 antibody A13963 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-11-07

Answer

The anti-BCAS4 antibody (A13963) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-11-07

Question

I was wanting to use your anti-BCAS4 antibody for WB for human lymph on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymph identification?

K. Huang

Verified customer

Asked: 2018-10-30

Answer

As indicated on the product datasheet, A13963 anti-BCAS4 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-10-30

Question

Does A13963 anti-BCAS4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-03-27

Answer

It shows on the product datasheet, A13963 anti-BCAS4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-03-27

Question

I see that the anti-BCAS4 antibody A13963 works with WB, what is the protocol used to produce the result images on the product page?

A. Moore

Verified customer

Asked: 2017-07-06

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-07-06

Question

Will anti-BCAS4 antibody A13963 work on canine WB with liver?

G. Brown

Verified customer

Asked: 2015-11-13

Answer

Our lab technicians have not validated anti-BCAS4 antibody A13963 on canine. You can run a BLAST between canine and the immunogen sequence of anti-BCAS4 antibody A13963 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-11-13

Order DetailsPrice
A13963-100uL

100uL

$360

Free Secondary Antibody

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A13963
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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