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Product Info Summary
| SKU: | EK2353 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Porcine |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma(heparin, EDTA) |
Product info
Product Name
Porcine IL-12/IL-23 p40 ELISA Kit PicoKine®
SKU/Catalog Number
EK2353
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Porcine IL-12/IL-23 p40 ELISA Kit PicoKine® (96 Tests). Quantitate Porcine IL-12/IL-23 p40 in cell culture supernatants, serum, plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Porcine IL-12/IL-23 p40 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2353)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Ile23-Asn324
Capture/Detection Antibodies
polyclonal antibody from goat|polyclonal antibody from goat
Sensitivity
<5 pg/mL
Assay Range
46.9-3,000 pg/mL
Standard Dilution Instructions
See datasheet of EK2353 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2353 is reactive to IL12B in Porcine samples
Validated Sample Types
cell culture supernatants, serum, plasma(heparin, EDTA)
Application Guarantee
EK2353 is guaranteed for ELISA in Porcine by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL12B
Subunit beta of interleukin 12 (IL12B), also known as interleukin 12B, or interleukin-12 subunit p40, is a subunit of human interleukin 12. It is mapped to 5q33.3. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2353-CAP | Anti-Porcine IL12B Pre-coated 96-well strip microplate | 1 |
| EK2353-ST | Porcine IL12B Standard | 2 vials, 10 ng/tube |
| EK2353-DA | Porcine IL12B Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.064 | 0.168 | 0.257 | 0.38 | 0.671 | 1.033 | 1.644 | 2.226 |
Data Example Images
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Porcine IL-12/IL-23 p40 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Porcine IL-12/IL-23 p40 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 59 | 248 | 822 | 88 | 333 | 1444 |
| Standard deviation | 4.57 | 17.36 | 18.66 | 4.75 | 25.17 | 91.84 |
| CV (%) | 7.7% | 7.0% | 2.3% | 5.4% | 7.6% | 6.4% |
Specific Publications For Porcine IL-12/IL-23 p40 ELISA Kit PicoKine® (EK2353)
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