Product Info Summary
SKU: | LS005467 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of peroxisome proliferator-activated receptor delta (PPARD), transcript variant 1 |
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Product info
Product Name
PPAR delta (PPARD) (NM_006238) Human Over-expression Lysate
See all PPAR delta/NR1C2 products
SKU/Catalog Number
LS005467
Size
100 µg
Description
Transient overexpression lysate of peroxisome proliferator-activated receptor delta (PPARD), transcript variant 1
Storage & Handling
Cite This Product
PPAR delta (PPARD) (NM_006238) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005467)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PPAR delta (PPARD) Human Over-expression Lysates NM_001171818.
Western blot validation of overexpression lysate (LS005467) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PPARD (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
PPAR delta (PPARD) Human Over-expression Lysates NM_001171819.
Western blot validation of overexpression lysate (LS005467) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PPARD (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
PPAR delta (PPARD) Human Over-expression Lysates NM_001171820.
Western blot validation of overexpression lysate (LS005467) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PPARD (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
PPAR delta (PPARD) Human Over-expression Lysates NM_006238.
Western blot validation of overexpression lysate (LS005467) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PPARD (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PPARD (Source: Uniprot.org, NCBI)
Gene Name
PPARD
Full Name
Peroxisome proliferator-activated receptor delta
Weight
49.903kDa
Superfamily
nuclear hormone receptor family
Alternative Names
FAAR; MGC3931; NR1C2; NR1C2PPAR-delta; NUC1; NUC1PPAR-beta; NUCI; NUCII; Nuclear hormone receptor 1; Nuclear receptor subfamily 1 group C member 2; peroxisome proliferative activated receptor, delta; Peroxisome proliferator-activated receptor beta; peroxisome proliferator-activated receptor delta; PPAR delta; PPARB; PPARD PPARD FAAR, NR1C2, NUC1, NUCI, NUCII, PPARB peroxisome proliferator activated receptor delta peroxisome proliferator-activated receptor delta|PPAR-beta|PPAR-delta|PPARD/MYO1D fusion|nuclear hormone receptor 1|nuclear receptor subfamily 1 group C member 2|peroxisome proliferator-activated receptor beta
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PPARD, check out the PPARD Infographic
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In this infographic, you will see the following information for PPARD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PPAR delta (PPARD) (NM_006238) Human Over-expression Lysate (LS005467)
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