Anti-PPARD Antibody (C-term)

PPAR delta/NR1C2 antibody

Boster Bio Anti-PPARD Antibody (C-term) (Catalog # A01557). Tested in WB application(s). This antibody reacts with Human.

Product Info Summary

SKU: A01557
Size: 80 µl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-PPARD Antibody (C-term)

View all PPAR delta/NR1C2 Antibodies

SKU/Catalog Number

A01557

Size

80 µl

Description

Boster Bio Anti-PPARD Antibody (C-term) (Catalog # A01557). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-PPARD Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01557)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This PPARD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from the C-terminal region of human PPARD.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01557 is reactive to PPARD in Human

Applications

A01557 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

49.903kDa

Background of PPAR delta/NR1C2

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation. Alternate splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For PPARD (Source: Uniprot.org, NCBI)

Gene Name

PPARD

Full Name

Peroxisome proliferator-activated receptor delta

Weight

49.903kDa

Superfamily

nuclear hormone receptor family

Alternative Names

FAAR; MGC3931; NR1C2; NR1C2PPAR-delta; NUC1; NUC1PPAR-beta; NUCI; NUCII; Nuclear hormone receptor 1; Nuclear receptor subfamily 1 group C member 2; peroxisome proliferative activated receptor, delta; Peroxisome proliferator-activated receptor beta; peroxisome proliferator-activated receptor delta; PPAR delta; PPARB; PPARD PPARD FAAR, NR1C2, NUC1, NUCI, NUCII, PPARB peroxisome proliferator activated receptor delta peroxisome proliferator-activated receptor delta|PPAR-beta|PPAR-delta|PPARD/MYO1D fusion|nuclear hormone receptor 1|nuclear receptor subfamily 1 group C member 2|peroxisome proliferator-activated receptor beta

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PPARD, check out the PPARD Infographic

PPARD infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PPARD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-PPARD Antibody (C-term)

Question

See attached the WB image, lot number and protocol we used for spleen using anti-PPAR delta antibody A01557. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-21

Question

Is this A01557 anti-PPAR delta antibody reactive to the isotypes of PPARD?

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

The immunogen of A01557 anti-PPAR delta antibody is Synthetic peptide with sequence CHPLLQEIYKDMY, corresponding to the C Terminus of the protein sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-23

Question

Is a blocking peptide available for product anti-PPAR delta antibody (A01557)?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

We do provide the blocking peptide for product anti-PPAR delta antibody (A01557). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-03

Question

We are currently using anti-PPAR delta antibody A01557 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-27

Answer

The anti-PPAR delta antibody (A01557) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-27

Question

Will anti-PPAR delta antibody A01557 work on feline IHC with placenta?

Verified Customer

Verified customer

Asked: 2019-08-14

Answer

Our lab technicians have not tested anti-PPAR delta antibody A01557 on feline. You can run a BLAST between feline and the immunogen sequence of anti-PPAR delta antibody A01557 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline placenta in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-14

Question

Is there a BSA free version of anti-PPAR delta antibody A01557 available?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PPAR delta antibody A01557 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-06-27

Question

Will anti-PPAR delta antibody A01557 work for WB with spleen?

B. Gonzalez

Verified customer

Asked: 2018-06-22

Answer

According to the expression profile of spleen, PPARD is highly expressed in spleen. So, it is likely that anti-PPAR delta antibody A01557 will work for WB with spleen.

Boster Scientific Support

Answered: 2018-06-22

Order DetailsPrice
A01557-80ul

80 µl

$298
A01557-400ul

400 µl

$460

Free Secondary Antibody

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A01557
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$298.00

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