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Product Info Summary
| SKU: | EK2375 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Primate |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma(heparin, EDTA) |
Product info
Product Name
Primate IFN-gamma ELISA Kit PicoKine®
SKU/Catalog Number
EK2375
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Primate IFN-gamma ELISA Kit PicoKine® (96 Tests). Quantitate Primate IFN-gamma in cell culture supernatants, serum, plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Primate IFN-gamma ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2375)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Gln24-Gln165
Capture/Detection Antibodies
polyclonal antibody from goat|polyclonal antibody from goat
Sensitivity
<5 pg/mL
Assay Range
31.3-2,000 pg/mL
Standard Dilution Instructions
See datasheet of EK2375 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2375 is reactive to IFNG in Primate samples
Validated Sample Types
cell culture supernatants, serum, plasma(heparin, EDTA)
Application Guarantee
EK2375 is guaranteed for ELISA in Primate by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IFNG
Interferon gamma receptor 1 (IFNGR1), also known as CD119 (Cluster of Differentiation 119), is a protein that in humans is encoded by the IFNGR1 gene. This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2375-CAP | Anti-Primate IFNG Pre-coated 96-well strip microplate | 1 |
| EK2375-ST | Primate IFNG Standard | 2 vials, 10 ng/tube |
| EK2375-DA | Primate IFNG Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.3 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.054 | 0.107 | 0.155 | 0.27 | 0.456 | 0.838 | 1.38 | 2.253 |
Data Example Images
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Primate IFN-gamma PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Primate IFN-gamma ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 60 | 125 | 732 | 36 | 195 | 553 |
| Standard deviation | 1.47 | 8.31 | 31.11 | 1.43 | 11.78 | 27.48 |
| CV (%) | 2.5% | 6.7% | 4.3% | 4.0% | 6.0% | 5.0% |
Specific Publications For Primate IFN-gamma ELISA Kit PicoKine® (EK2375)
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