Product Info Summary
SKU: | LS005573 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 1 |
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Product info
Product Name
Protein Kinase A regulatory subunit I alpha (PRKAR1A) (NM_002734) Human Over-expression Lysate
See all Protein Kinase A regulatory subunit I alpha products
SKU/Catalog Number
LS005573
Size
100 µg
Description
Transient overexpression lysate of protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 1
Storage & Handling
Cite This Product
Protein Kinase A regulatory subunit I alpha (PRKAR1A) (NM_002734) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005573)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Protein Kinase A regulatory subunit I alpha (PRKAR1A) Human Over-expression Lysates NM_002734.
Western blot validation of overexpression lysate (LS005573) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PRKAR1A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
Protein Kinase A regulatory subunit I alpha (PRKAR1A) Human Over-expression Lysates NM_212471.
Western blot validation of overexpression lysate (LS005573) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PRKAR1A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
Protein Kinase A regulatory subunit I alpha (PRKAR1A) Human Over-expression Lysates NM_212471.
Western blot validation of overexpression lysate (LS005573) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PRKAR1A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
Protein Kinase A regulatory subunit I alpha (PRKAR1A) Human Over-expression Lysates NM_212472.
Western blot validation of overexpression lysate (LS005573) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PRKAR1A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PRKAR1A (Source: Uniprot.org, NCBI)
Gene Name
PRKAR1A
Full Name
cAMP-dependent protein kinase type I-alpha regulatory subunit
Weight
42.982kDa
Superfamily
cAMP-dependent kinase regulatory chain family
Alternative Names
cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain; cAMP-dependent protein kinase type I-alpha regulatory subunit; CAR; CNC; CNC1; MGC17251; PKA R1 alpha; PKA1; PKR1; PPNAD1; PRKAR1; protein kinase A type 1a regulatory subunit; protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specificextinguisher 1); Tissue-specific extinguisher 1; TSE1DKFZp779L0468 PRKAR1A ACRDYS1, ADOHR, CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, TSE1 protein kinase cAMP-dependent type I regulatory subunit alpha cAMP-dependent protein kinase type I-alpha regulatory subunit|Carney complex type 1|cAMP-dependent protein kinase regulatory subunit RIalpha|cAMP-dependent protein kinase type I-alpha regulatory chain|epididymis secretory sperm binding protein|protein kinase A type 1a regulatory subunit|protein kinase, cAMP-dependent, regulatory subunit type I alpha|protein kinase, cAMP-dependent, regulatory, type I, alpha|tissue-specific extinguisher 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PRKAR1A, check out the PRKAR1A Infographic
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In this infographic, you will see the following information for PRKAR1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Protein Kinase A regulatory subunit I alpha (PRKAR1A) (NM_002734) Human Over-expression Lysate (LS005573)
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