KAP0 Colorimetric Cell-Based ELISA

The KAP0 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor KAP0 protein expression profile in cells. The kit can be used for measuring the relative amounts of KAP0 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on KAP0.

Product Info Summary

SKU: EKC1676
Size: 1 kit, containing 1 plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

KAP0 Colorimetric Cell-Based ELISA

See all Protein Kinase A regulatory subunit I alpha products

SKU/Catalog Number

EKC1676

Size

1 kit, containing 1 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The KAP0 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor KAP0 protein expression profile in cells. The kit can be used for measuring the relative amounts of KAP0 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on KAP0.

Storage & Handling

4°C/6 Months

Cite This Product

KAP0 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1676)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC1676 is reactive to PRKAR1A in Human, Mouse, Rat samples

Application Guarantee

EKC1676 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Protein Kinase A regulatory subunit I alpha Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For PRKAR1A (Source: Uniprot.Org, NCBI)

Uniprot ID

P10644

Gene ID

5573

Gene Name

PRKAR1A

Full Name

cAMP-dependent protein kinase type I-alpha regulatory subunit

Weight

42.982kDa

Superfamily

cAMP-dependent kinase regulatory chain family

Alternative Names

cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain; cAMP-dependent protein kinase type I-alpha regulatory subunit; CAR; CNC; CNC1; MGC17251; PKA R1 alpha; PKA1; PKR1; PPNAD1; PRKAR1; protein kinase A type 1a regulatory subunit; protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specificextinguisher 1); Tissue-specific extinguisher 1; TSE1DKFZp779L0468 PRKAR1A ACRDYS1, ADOHR, CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, TSE1 protein kinase cAMP-dependent type I regulatory subunit alpha cAMP-dependent protein kinase type I-alpha regulatory subunit|Carney complex type 1|cAMP-dependent protein kinase regulatory subunit RIalpha|cAMP-dependent protein kinase type I-alpha regulatory chain|epididymis secretory sperm binding protein|protein kinase A type 1a regulatory subunit|protein kinase, cAMP-dependent, regulatory subunit type I alpha|protein kinase, cAMP-dependent, regulatory, type I, alpha|tissue-specific extinguisher 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PRKAR1A, check out the PRKAR1A Infographic

PRKAR1A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PRKAR1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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