Rabbit N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®

N-Cadherin ELISA kit for Rabbit

Rabbit N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine™ (96 Tests). Quantitate Rabbit CDH2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Product Info Summary

SKU: EK0669-RB
Size: 96 wells/kit, with removable strips.
Reactive Species: Rabbit
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Rabbit N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®

View all N-Cadherin ELISA kits

SKU/Catalog Number

EK0669-RB

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rabbit N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine™ (96 Tests). Quantitate Rabbit CDH2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rabbit N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0669-RB)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: D160-A724

Sensitivity

<20 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0669-RB for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0669-RB is reactive to CDH2 in Rabbit samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK0669-RB is guaranteed for ELISA in Rabbit by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of N-Cadherin

Cadherin-2 (CDH2), also known as neural cadherin (NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Kit Components

Catalog Number Description Quantity
EK0669-RB-CAP Anti-Rabbit CDH2 Pre-coated 96-well strip microplate 1
EK0669-RB-ST Rabbit CDH2 Standard 2 vials, 10 ng/tube
EK0669-RB-DA Rabbit CDH2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0250.0750.1190.2100.3690.7491.3552.007

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rabbit N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (ng/ml)1.233.466.921.383.576.85
Standard deviation0.0590.1800.3880.0730.2030.425
CV (%)4.85.25.65.35.76.2

Gene/Protein Information For CDH2 (Source: Uniprot.Org, NCBI)

Gene Name

CDH2

Full Name

Cadherin-2

Weight

99.809kDa

Alternative Names

cadherin 2, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; CD325 antigen; CD325; CDH2; CDHNcalcium-dependent adhesion protein, neuronal; CDw325; NCadherin; N-Cadherin; NCADN-cadherin 1; Neural cadherin; neural-cadherin CDH2 ACOGS, ARVD14, CD325, CDHN, CDw325, NCAD cadherin 2 cadherin-2|N-cadherin 1|cadherin 2, type 1, N-cadherin (neuronal)|calcium-dependent adhesion protein, neuronal|neural cadherin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDH2, check out the CDH2 Infographic

CDH2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDH2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EK0669-RB

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Rabbit N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

Be the first to review Rabbit N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

8 Customer Q&As for Rabbit N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®

Question

Q: can I use citrate plasma as samples in Rabbit N-Cadherin Picokine® ELISA Kit (Catalog # EK0669-RB)?

S. Jones

Verified customer

Asked: 2020-06-06

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of N-Cadherin causing degradation of its protein structure. N-Cadherin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the N-Cadherin ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-06-06

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-01-08

Question

Q: if the enzyme conjugated N-Cadherin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the N-Cadherin antigen?

H. Walker

Verified customer

Asked: 2019-06-09

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-06-09

Question

Q: how to thaw whole blood sample for N-Cadherin ELISA after freezing?

Z. Davis

Verified customer

Asked: 2018-12-17

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test N-Cadherin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-12-17

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-03-21

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-03-21

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-12-09

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-12-09

Question

Q: What is the optimal O.D. value for N-Cadherin ELISA kit? I used your N-Cadherin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain N-Cadherin even though the O.D. values are not very high?

N. Mangal

Verified customer

Asked: 2016-10-11

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this N-Cadherin ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of N-Cadherin that can be declared/interpreted as positive by the above standard is 20pg/ml.

Boster Scientific Support

Answered: 2016-10-11

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of N-Cadherin in Rabbit serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2014-10-14

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the N-Cadherin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2014-10-14

$499
Backordered.

Lead time for this item is typically 2-3 weeks

Get A Quote
In stock
Order Product
EK0669-RB
$499.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.