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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Rabbit SFRP5 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rabbit SFRP5 ELISA Kit PicoKine™ (96 Tests). Quantitate Rabbit SFRP5 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rabbit SFRP5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1472-RB)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: CHO; Immunogen sequence: E30-H317
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1472-RB is reactive to SFRP5 in Rabbit samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1472-RB is guaranteed for ELISA in Rabbit by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of sFRP-5
Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.
|EK1472-RB-CAP||Anti-Rabbit SFRP5 Pre-coated 96-well strip microplate||1|
|EK1472-RB-ST||Rabbit SFRP5 Standard||2 vials, 10 ng/tube|
|EK1472-RB-DA||Rabbit SFRP5 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rabbit SFRP5 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rabbit SFRP5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
Gene/Protein Information For SFRP5 (Source: Uniprot.Org, NCBI)
Secreted frizzled-related protein 5
secreted frizzled-related protein (sFRP) family
frizzled-related protein 1b; FRP1B; FRP-1b; SARP3; SARP-3; secreted apoptosis related protein 3; secreted apoptosis-related protein 3; secreted frizzled-related protein 5; sFRP5; sFRP-5 SFRP5 SARP3 secreted frizzled related protein 5 secreted frizzled-related protein 5|FRP-1b|SARP-3|frizzled-related protein 1b|sFRP-5|secreted apoptosis related protein 3*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on SFRP5, check out the SFRP5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SFRP5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK1472-RB
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