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Product Info Summary
| SKU: | EK2271 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat CD25/IL2RA ELISA Kit PicoKine®
View all CD25/IL-2R alpha ELISA kits
SKU/Catalog Number
EK2271
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat CD25/IL2RA ELISA Kit PicoKine® (96 Tests). Quantitate Rat CD25/IL2RA in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 45 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat CD25/IL2RA ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2271)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: E22-Q235
Sensitivity
<45 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2271 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2271 is reactive to IL2RA in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2271 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD25/IL-2R alpha
CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2271-CAP | Anti-Rat IL2RA Pre-coated 96-well strip microplate | 1 |
| EK2271-ST | Rat IL2RA Standard | 2 vials, 10 ng/tube |
| EK2271-DA | Rat IL2RA Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1,500 | 3,000 | 6,000 |
| O.D. | 0.019 | 0.084 | 0.156 | 0.395 | 0.581 | 0.881 | 1.275 | 1.831 |
Data Example Images
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Rat CD25 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat CD25/IL2RA ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 114 | 306 | 2233 | 155 | 316 | 2793 |
| Standard deviation | 6.68 | 15.67 | 64.09 | 10.37 | 22.03 | 161.99 |
| CV(%) | 5.9% | 5.1% | 2.9% | 6.7% | 7% | 5.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD25/IL-2R alpha in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 114 | 154 | 167 | 139 | 144 | 4.69 | 3.3% |
| Sample 2 | 306 | 243 | 257 | 203 | 252 | 19.55 | 7.8% |
| Sample 3 | 2233 | 2818 | 2456 | 2488 | 2499 | 151.67 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For IL2RA (Source: Uniprot.Org, NCBI)
Gene Name
IL2RA
Full Name
Interleukin-2 receptor subunit alpha
Weight
30.819kDa
Alternative Names
CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR IL2RA CD25, IDDM10, IL2R, IMD41, TCGFR, p55 interleukin 2 receptor subunit alpha interleukin-2 receptor subunit alpha|IL-2 receptor subunit alpha|IL-2R subunit alpha|TAC antigen|interleukin 2 receptor, alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL2RA, check out the IL2RA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL2RA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat CD25/IL2RA ELISA Kit PicoKine® (EK2271)
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