Product Info Summary
SKU: | EK1816 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Rat |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). |
Product info
Product Name
Rat Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine®
SKU/Catalog Number
EK1816
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ces1d in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1816)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Y19-L565
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1816 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1816 is reactive to Ces1d in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
Application Guarantee
EK1816 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Ces1d
This gene is mapped to 19p11. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. Enzyme that hydrolizes long-chain fatty acids and thioesters; may play a role in lipid metabolism and/or the elimination of toxic substances. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK1816-CAP | Anti-Rat Ces1d Pre-coated 96-well strip microplate | 1 |
EK1816-ST | Rat Ces1d Standard | 2 vials, 10 ng/tube |
EK1816-DA | Rat Ces1d Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.018 | 0.170 | 0.291 | 0.609 | 1.135 | 1.763 | 2.142 | 2.383 |
Data Example Images
Click image to see more details
Rat ES3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Ces1d, Dilution ratio of 1:1, concentration in serum and plasma is around 2 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 226 | 1074 | 4954 | 239 | 1164 | 4979 |
Standard deviation | 11.98 | 59.07 | 322.01 | 10.99 | 80.32 | 383.4 |
CV (%) | 5.3% | 5.5% | 6.5% | 4.6% | 6.9% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Ces1d in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 226 | 230 | 236 | 230 | 230 | 3.57 | 1.5% |
Sample 2 | 1074 | 1096 | 1126 | 1007 | 1075 | 43.77 | 4.0% |
Sample 3 | 4954 | 4898 | 5293 | 4648 | 4948 | 229.97 | 4.6% |
Protein Target Info & Infographic
Gene/Protein Information For Ces1d (Source: Uniprot.Org, NCBI)
Gene Name
Ces1d
Full Name
Carboxylesterase 1D
Weight
61.788kDa
Superfamily
type-B carboxylesterase/lipase family
Alternative Names
Carboxylesterase 1D Ces1d|Ces, Ces3, TGH|carboxylesterase 1D|carboxylesterase 1D|FAEE synthase|carboxylesterase 3|fatty acid ethyl ester synthase|triacylglycerol hydrolase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Ces1d, check out the Ces1d Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ces1d: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine® (EK1816)
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No publications found for EK1816
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Rat Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
S. Allen
Verified customer
Asked: 2020-12-06
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-12-06
Question
Q: if the enzyme conjugated Ces1d antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Ces1d antigen?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-01-30
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Ces1d using the EK1816 Rat Ces1d Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-10-19
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2019-10-19
Question
Q: can you suggest the dilution ratio of serum samples for detection of Ces1d in Rat plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
E. Huang
Verified customer
Asked: 2019-07-26
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Ces1d ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-07-26
Question
Q: What is the optimal O.D. value for Ces1d ELISA kit? I used your Ces1d ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Ces1d even though the O.D. values are not very high?
M. Mitchell
Verified customer
Asked: 2019-01-14
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Ces1d ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Ces1d that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2019-01-14
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-11-24
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-11-24
Question
Q: is it okay to use citrate plasma as samples in Rat Ces1d Picokine® ELISA Kit (Catalog # EK1816)?
Verified Customer
Verified customer
Asked: 2018-04-28
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Ces1d causing disruption of its protein structure. Ces1d may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Ces1d ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-04-28
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
R. Puri
Verified customer
Asked: 2015-04-06
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2015-04-06