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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).|
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Rat Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ces1d in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1816)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Y19-L565
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1816 is reactive to Ces1d in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
EK1816 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Ces1d
This gene is mapped to 19p11. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. Enzyme that hydrolizes long-chain fatty acids and thioesters; may play a role in lipid metabolism and/or the elimination of toxic substances. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined.
|EK1816-CAP||Anti-Rat Ces1d Pre-coated 96-well strip microplate||1|
|EK1816-ST||Rat Ces1d Standard||2 vials, 10 ng/tube|
|EK1816-DA||Rat Ces1d Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat ES3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Ces1d, Dilution ratio of 1:1, concentration in serum is around 2ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Ces1d in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Ces1d (Source: Uniprot.Org, NCBI)
type-B carboxylesterase/lipase family
Carboxylesterase 1D Ces1d|Ces3|carboxylesterase 1D|carboxylesterase 1D|ES-HVEL|FAEE synthase|carboxyesterase ES-10|carboxylesterase 3|fatty acid ethyl ester synthase|liver carboxylesterase 10|pI 6.1 esterase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ces1d, check out the Ces1d Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ces1d: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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