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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Rat Contactin-1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat Contactin-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Cntn1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Contactin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1773)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: E21-S1001
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1773 is reactive to Cntn1 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1773 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Contactin-1
Contactin 1, also known as CNTN1, is a protein which in humans is encoded by the CNTN1 gene. It is mapped to 7q35. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.
|EK1773-CAP||Anti-Rat Cntn1 Pre-coated 96-well strip microplate||1|
|EK1773-ST||Rat Cntn1 Standard||2 vials, 10 ng/tube|
|EK1773-DA||Rat Cntn1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat Contactin-1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Contactin-1, Dilution ratio of 1:10, concentration in serum is around 23ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Contactin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Contactin-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Cntn1 (Source: Uniprot.Org, NCBI)
CNTN1; contactin 1; Contactin1; Contactin-1; F11; F3; F3cam; Glycoprotein gp135; GP135; Neural cell surface protein F3 Cntn1|F3|contactin 1|contactin-1|neural cell surface protein F3*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cntn1, check out the Cntn1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cntn1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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