Rat Cystatin C/CST3 ELISA Kit PicoKine™

Rat Cystatin C/CST3 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Cst3 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK1109
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Rat Cystatin C/CST3 ELISA Kit PicoKine™

See all Cystatin C products

SKU/Catalog Number

EK1109

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat Cystatin C/CST3 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Cst3 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat Cystatin C/CST3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1109)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-A140

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1109 is reactive to Cst3 in Rat samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK1109 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1109-CAP Anti-Rat Cst3 Pre-coated 96-well strip microplate 1
EK1109-ST Rat Cst3 Standard 2 vials, 20 ng/tube
EK1109-DA Rat Cst3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0090.2850.5250.8161.2221.3681.5871.951

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat Cystatin C/CST3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)8242517810475625567350
Standard deviation34.6128.36445.7239.31146.49470.40
CV (%)4.2%6.4%5.5%5.2%5.7%6.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cystatin C in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 182479378573578431.944%
Sample 22517269326422687263470.782.6%
Sample 38104801482438197813988.071%
*number of samples for each test n=16.

Gene/Protein Information For Cst3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P14841

Gene ID

25307

Gene Name

Cst3

Full Name

Cystatin-C

Weight

15.437kDa

Superfamily

cystatin family

Alternative Names

ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin Cst3|CYSC|cystatin C|cystatin-C|Cystatin C (cysteine proteinase inhibitor)|cystatin-3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cst3, check out the Cst3 Infographic

Cst3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cst3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1109 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The effect of trimetazidine on renal oxidative stress in partial and complete ureteral obstructions in a rat model

Benli E, Ayyildiz SN, Cirrik S, Kokt

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6 Customer Q&As for Rat Cystatin C/CST3 ELISA Kit PicoKine™

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Cystatin C using the EK1109 Rat Cystatin C Picokine® ELISA Kit?

T. Johnson

Verified customer

Asked: 2020-11-09

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2020-11-09

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Cystatin C in Rat serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

H. Clark

Verified customer

Asked: 2020-05-19

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Cystatin C ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-05-19

Question

Q: can I use citrate plasma as samples in Rat Cystatin C Picokine® ELISA Kit (Catalog # EK1109)?

Verified Customer

Verified customer

Asked: 2017-11-19

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Cystatin C causing degradation of its protein structure. Cystatin C may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Cystatin C ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-11-19

Question

Q: which procedure should I follow in order to thaw whole blood sample for Cystatin C ELISA after freezing?

Verified Customer

Verified customer

Asked: 2017-09-28

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Cystatin C for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2017-09-28

Question

Q: if the enzyme conjugated Cystatin C antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cystatin C antigen?

T. White

Verified customer

Asked: 2017-05-06

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-05-06

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

L. Young

Verified customer

Asked: 2016-02-10

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-02-10

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