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Product Info Summary
| SKU: | EK1573 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. |
Product info
Product Name
Rat FABP4/A Fabp ELISA Kit PicoKine®
View all FABP4/A-FABP ELISA kits
SKU/Catalog Number
EK1573
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat FABP4/A Fabp ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Fabp4 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat FABP4/A Fabp ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1573)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: M1-A132
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1573 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1573 is reactive to FABP4 in Rat samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.
Application Guarantee
EK1573 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FABP4/A-FABP
FABP4, also known as aP2 (adipocyte Protein 2), encodes the fatty acid binding protein found in adipocytes. The FABP4 gene is mapped to 8q21. FABP4 functions as a cytosolic lipid chaperone in macrophages and is involved in regulating macrophage endoplasmic reticulum (ER) stress. Aditionally, FABP4 protein is expressed in HBEs and is strongly upregulated by both IL4 and IL13 and downregulated by IFN-gamma, suggesting a role in allergic airway inflammation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1573-CAP | Anti-Rat FABP4 Pre-coated 96-well strip microplate | 1 |
| EK1573-ST | Rat FABP4 Standard | 2 vials, 10 ng/tube |
| EK1573-DA | Rat FABP4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.084 | 0.129 | 0.171 | 0.233 | 0.391 | 0.712 | 1.320 | 2.040 |
Data Example Images
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Rat FABP4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FABP4/A-FABP, Dilution ratio of 1:50, concentration in serum and plasma is around 50 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat FABP4/A Fabp ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 290 | 1382 | 5455 | 295 | 1277 | 5547 |
| Standard deviation | 13.05 | 82.92 | 223.65 | 14.45 | 80.45 | 266.25 |
| CV (%) | 4.5% | 6% | 4.1% | 4.9% | 6.3% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FABP4/A-FABP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 290 | 325 | 321 | 286 | 305 | 17.61 | 5.7% |
| Sample 2 | 1382 | 1337 | 1562 | 1377 | 1414 | 86.92 | 6.1% |
| Sample 3 | 5455 | 5227 | 5317 | 5215 | 5303 | 95.94 | 1.8% |
Protein Target Info & Infographic
Gene/Protein Information For FABP4 (Source: Uniprot.Org, NCBI)
Gene Name
FABP4
Full Name
Fatty acid-binding protein, adipocyte
Weight
14.719kDa
Superfamily
calycin superfamily
Alternative Names
Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; AFABP; A-FABP; A-FABPAFABP; ALBP; aP2; FABP4; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; fatty acid-binding protein, adipocyte FABP4 A-FABP, AFABP, ALBP, HEL-S-104, aP2 fatty acid binding protein 4 fatty acid-binding protein, adipocyte|adipocyte fatty acid binding protein|adipocyte lipid-binding protein|adipocyte-type fatty acid-binding protein|epididymis secretory protein Li 104|fatty acid binding protein 4, adipocyte
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FABP4, check out the FABP4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FABP4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat FABP4/A Fabp ELISA Kit PicoKine® (EK1573)
Hello CJ!
EK1573 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Adipokines and Metabolic Regulators in Human and Experimental Pulmonary Arterial Hypertension
FABP4 contributes to renal interstitial fibrosis via mediating inflammation and lipid metabolism
Papathanasiou AE,Spyropoulos F,Michael Z,Joung KE,Briana DD,Malamitsi-Puchner A,Mantzoros CS,Christou H.Adipokines and Metabolic Regulators in Human and Experimental Pulmonary Arterial Hypertension.Int J Mol Sci.2021 Feb 1;22(3):1435.doi:10.3390/ijms22031
Species: Human,Rat
EK1573 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Rat FABP4/A Fabp ELISA Kit PicoKine®
Question
Q: Can FABP4 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
K. Thompson
Verified customer
Asked: 2020-08-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-08-16
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
S. Mitchell
Verified customer
Asked: 2020-03-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-03-22
Question
Q: how can I thaw whole blood sample for FABP4 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FABP4 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-02-25
Question
Q: What is the optimal O.D. value for FABP4 ELISA kit? I performed your FABP4 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain FABP4 even though the O.D. values are not very high?
J. Kapoor
Verified customer
Asked: 2019-02-18
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this FABP4 ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of FABP4 that can be declared/interpreted as positive by the above standard is 15pg/ml.
Boster Scientific Support
Answered: 2019-02-18
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
K. Maharaj
Verified customer
Asked: 2019-02-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-02-08
Question
Q: how to proceed with the analysis of ELISA data? I measured FABP4 level in cell lysates.
Verified Customer
Verified customer
Asked: 2015-03-05
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2015-03-05
Question
Q: is it okay to use citrate plasma as samples in Rat FABP4 Picokine® ELISA Kit (Catalog # EK1573)?
R. Chakrabarti
Verified customer
Asked: 2014-10-17
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of FABP4 causing disruption of its protein structure. FABP4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the FABP4 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2014-10-17
