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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Rat FGF23 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat FGF23 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Fgf23 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml .
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Rat FGF23 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1591)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: Y25-V251
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1591 is reactive to Fgf23 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1591 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1591-CAP||Anti-Rat Fgf23 Pre-coated 96-well strip microplate||1|
|EK1591-ST||Rat Fgf23 Standard||2 vials, 10 ng/tube|
|EK1591-DA||Rat Fgf23 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat FGF23 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FGF-23, Dilution ratio of 1:1, concentration in serum is around 400pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat FGF23 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of FGF-23 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
|Sample . 2||244||245||238||254||245||5.71||2.3%|
Gene/Protein Information For Fgf23 (Source: Uniprot.Org, NCBI)
Fibroblast growth factor 23
heparin-binding growth factors family
ADHR; FGF23; FGF-23; fibroblast growth factor 23; HPDR2; HYPF; phosphatonin; PHPTC; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor Fgf23|Fgf8b|fibroblast growth factor 23|fibroblast growth factor 23|FGF-23|fibroblast growth factor 8b*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Fgf23, check out the Fgf23 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Fgf23: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK1591
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