Rat FGFBP1 ELISA Kit PicoKine™

Rat FGFBP1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Fgfbp1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml .

Product Info Summary

SKU: EK1695
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Rat FGFBP1 ELISA Kit PicoKine™

See all FGF-BP products

SKU/Catalog Number

EK1695

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat FGFBP1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Fgfbp1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml .

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat FGFBP1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1695)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-C238

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1695 is reactive to Fgfbp1 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1695 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1695-CAP Anti-Rat Fgfbp1 Pre-coated 96-well strip microplate 1
EK1695-ST Rat Fgfbp1 Standard 2 vials, 10 ng/tube
EK1695-DA Rat Fgfbp1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156313625125025005000
O.D.0.0660.1560.2110.3360.5650.9911.7412.475

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect FGF-BP, Dilution ratio of 1:1, concentration in serum is around 800pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat FGFBP1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)11256626621196072774
Standard deviation6.936.79114.476.1937.03199.7
CV (%)6.2%6.5%4.3%5.2%6.1%7.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of FGF-BP in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11121081171121123.192.8%
Sample . 256662967461162038.716.2%%
Sample 326622454276528012670135.005.0%
*number of samples for each test n=16.

Gene/Protein Information For Fgfbp1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9QY10

Gene ID

64535

Gene Name

Fgfbp1

Full Name

Fibroblast growth factor-binding protein 1

Weight

26.887kDa

Superfamily

fibroblast growth factor-binding protein family

Alternative Names

17 kDa heparin-binding growth factor-binding protein; FGFBP; FGF-BP; FGFBP1; FGF-BP1; FGFBP-1; FGFBPFGF-binding protein 1; fibroblast growth factor binding protein 1; fibroblast growth factor-binding protein 1; HBp17; HBP1717 kDa HBGF-binding protein; heparin-binding growth factor binding protein Fgfbp1|fibroblast growth factor binding protein 1|fibroblast growth factor-binding protein 1|FGF-BP|FGF-BP1|FGF-binding protein 1|FGFBP-1|growth factor-binding protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Fgfbp1, check out the Fgfbp1 Infographic

Fgfbp1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Fgfbp1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Rat FGFBP1 ELISA Kit PicoKine™

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

D. Baker

Verified customer

Asked: 2019-09-16

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-09-16

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of FGF-BP in Rat serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-06-01

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FGF-BP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-06-01

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-05-19

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-05-19

Question

Q: how do I analyze ELISA data? I measured FGF-BP level in serum.

Verified Customer

Verified customer

Asked: 2016-08-14

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2016-08-14

Question

Q: is it okay to use citrate plasma as samples in Rat FGF-BP Picokine® ELISA Kit (Catalog # EK1695)?

Verified Customer

Verified customer

Asked: 2016-06-09

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of FGF-BP causing disruption of its protein structure. FGF-BP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the FGF-BP ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-06-09

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