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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and serum.|
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Rat Flt-3 ligand ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat Flt-3 ligand ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Flt3lg in cell culture supernatants and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Rat Flt-3 ligand ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2101)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: G27-R188
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK2101 is reactive to Flt3lg in Rat samples
Validated Sample Types
cell culture supernatants and serum.
EK2101 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK2101-CAP||Anti-Rat Flt3lg Pre-coated 96-well strip microplate||1|
|EK2101-ST||Rat Flt3lg Standard||2 vials, 10 ng/tube|
|EK2101-DA||Rat Flt3lg Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat Flt-3 ligand PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Flt-3 Ligand/FLT3L, Dilution ratio of 1:1, concentration in serum is around 500pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Flt-3 ligand ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Flt-3 Ligand/FLT3L in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Flt3lg (Source: Uniprot.Org, NCBI)
Fms-related tyrosine kinase 3 ligand
FL; FLG3L; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine FLT3LG FL, FLG3L, FLT3L fms related receptor tyrosine kinase 3 ligand fms-related tyrosine kinase 3 ligand|flt3 ligand|fms related tyrosine kinase 3 ligand*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Flt3lg, check out the Flt3lg Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Flt3lg: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK2101
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