Rat Flt-3 ligand ELISA Kit PicoKine™

Rat Flt-3 ligand ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Flt3lg in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK2101
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Rat Flt-3 ligand ELISA Kit PicoKine™

See all Flt-3 Ligand/FLT3L products

SKU/Catalog Number

EK2101

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat Flt-3 ligand ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Flt3lg in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat Flt-3 ligand ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2101)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: G27-R188

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2101 is reactive to Flt3lg in Rat samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK2101 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK2101-CAP Anti-Rat Flt3lg Pre-coated 96-well strip microplate 1
EK2101-ST Rat Flt3lg Standard 2 vials, 10 ng/tube
EK2101-DA Rat Flt3lg Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0300.1170.1990.3230.5900.9391.6542.280

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Flt-3 Ligand/FLT3L, Dilution ratio of 1:1, concentration in serum is around 500pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat Flt-3 ligand ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3616069941184759
Standard deviation1.626.8828.662.4610.6749.34
CV (%)4.5%4.3%4.1%6.0%5.8%6.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Flt-3 Ligand/FLT3L in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 136373334351.584.5%
Sample 216016218418217211.046.4%
Sample 369968062163765931.494.7%
*number of samples for each test n=16.

Gene/Protein Information For Flt3lg (Source: Uniprot.Org, NCBI)

Uniprot ID

P49771

Gene ID

2323

Gene Name

Flt3lg

Full Name

Fms-related tyrosine kinase 3 ligand

Weight

26.416kDa

Alternative Names

FL; FLG3L; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine FLT3LG FL, FLG3L, FLT3L fms related receptor tyrosine kinase 3 ligand fms-related tyrosine kinase 3 ligand|flt3 ligand|fms related tyrosine kinase 3 ligand

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Flt3lg, check out the Flt3lg Infographic

Flt3lg infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Flt3lg: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Rat Flt-3 ligand ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated Flt-3 Ligand antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Flt-3 Ligand antigen?

Verified Customer

Verified customer

Asked: 2020-11-04

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-11-04

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-12-19

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-12-19

Question

Q: What is the optimal O.D. value for Flt-3 Ligand ELISA kit? I performed your Flt-3 Ligand ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Flt-3 Ligand even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-11-05

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Flt-3 Ligand ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Flt-3 Ligand that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-11-05

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Flt-3 Ligand using the EK2101 Rat Flt-3 Ligand Picokine® ELISA Kit?

N. Thomas

Verified customer

Asked: 2018-08-07

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2018-08-07

Question

Q: can you suggest the dilution ratio of serum samples for detection of Flt-3 Ligand in Rat serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

W. Brown

Verified customer

Asked: 2016-06-30

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Flt-3 Ligand ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2016-06-30

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2016-05-14

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-05-14

Question

Q: which procedure should I follow in order to thaw whole blood sample for Flt-3 Ligand ELISA after freezing?

H. Edwards

Verified customer

Asked: 2014-09-11

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Flt-3 Ligand for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2014-09-11

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