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Product Info Summary
| SKU: | EK2101 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Rat Flt-3 ligand ELISA Kit PicoKine®
View all Flt-3 Ligand/FLT3L ELISA kits
SKU/Catalog Number
EK2101
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Flt-3 ligand ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Flt3lg in cell culture supernatants and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Flt-3 ligand ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2101)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: G27-R188
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2101 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2101 is reactive to FLT3LG in Rat samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK2101 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Flt-3 Ligand/FLT3L
Fms-related tyrosine kinase 3 ligand (FLT3LG) is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 1q22. Flt3 ligand (FL) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. For example, it is a major growth factor stimulating the growth of dendritic cells.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2101-CAP | Anti-Rat FLT3LG Pre-coated 96-well strip microplate | 1 |
| EK2101-ST | Rat FLT3LG Standard | 2 vials, 10 ng/tube |
| EK2101-DA | Rat FLT3LG Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.030 | 0.117 | 0.199 | 0.323 | 0.590 | 0.939 | 1.654 | 2.280 |
Data Example Images
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Rat Flt-3 ligand PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Flt-3 Ligand/FLT3L, Dilution ratio of 1:1, concentration in serum and plasma is around 500 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Flt-3 ligand ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 36 | 160 | 699 | 41 | 184 | 759 |
| Standard deviation | 1.62 | 6.88 | 28.66 | 2.46 | 10.67 | 49.34 |
| CV (%) | 4.5% | 4.3% | 4.1% | 6.0% | 5.8% | 6.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Flt-3 Ligand/FLT3L in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 36 | 37 | 33 | 34 | 35 | 1.58 | 4.5% |
| Sample 2 | 160 | 162 | 184 | 182 | 172 | 11.04 | 6.4% |
| Sample 3 | 699 | 680 | 621 | 637 | 659 | 31.49 | 4.7% |
Protein Target Info & Infographic
Gene/Protein Information For FLT3LG (Source: Uniprot.Org, NCBI)
Gene Name
FLT3LG
Full Name
Fms-related tyrosine kinase 3 ligand
Weight
26.416kDa
Alternative Names
FL; FLG3L; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine FLT3LG FL, FLG3L, FLT3L fms related receptor tyrosine kinase 3 ligand fms-related tyrosine kinase 3 ligand|flt3 ligand|fms related tyrosine kinase 3 ligand
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FLT3LG, check out the FLT3LG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FLT3LG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat Flt-3 ligand ELISA Kit PicoKine® (EK2101)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Rat Flt-3 ligand ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated Flt-3 Ligand antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Flt-3 Ligand antigen?
Verified Customer
Verified customer
Asked: 2020-11-04
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-04
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-12-19
Question
Q: What is the optimal O.D. value for Flt-3 Ligand ELISA kit? I performed your Flt-3 Ligand ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Flt-3 Ligand even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-11-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Flt-3 Ligand ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Flt-3 Ligand that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-11-05
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Flt-3 Ligand using the EK2101 Rat Flt-3 Ligand Picokine® ELISA Kit?
N. Thomas
Verified customer
Asked: 2018-08-07
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-08-07
Question
Q: can you suggest the dilution ratio of serum samples for detection of Flt-3 Ligand in Rat serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
W. Brown
Verified customer
Asked: 2016-06-30
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Flt-3 Ligand ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2016-06-30
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2016-05-14
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-05-14
Question
Q: which procedure should I follow in order to thaw whole blood sample for Flt-3 Ligand ELISA after freezing?
H. Edwards
Verified customer
Asked: 2014-09-11
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Flt-3 Ligand for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2014-09-11
