Anti-Flt3 ligand/FLT3LG Antibody

Flt-3 Ligand/FLT3L antibody

Boster Bio Anti-Flt3 ligand/FLT3LG Antibody catalog # PA1914. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA1914
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Flt3 ligand/FLT3LG Antibody

View all Flt-3 Ligand/FLT3L Antibodies

SKU/Catalog Number

PA1914

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Flt3 ligand/FLT3LG Antibody catalog # PA1914. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Flt3 ligand/FLT3LG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1914)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Flt-3ligand (206-220aa HWQRTRRRTPRPGEQ).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1914 is reactive to FLT3LG in Human

Applications

PA1914 is guaranteed for WB Boster Guarantee

Background of Flt-3 Ligand/FLT3L

FLT3LG (FMS-Related Tyrosine Kinase 3 Ligand) also called FLT3 LIGAND, FL or FLT3L, is a protein which in humans is encoded by the FLT3LG gene. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt3 ligand (FL) is a hematopoietic four helical bundlecytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. Lyman et al. (1993) found that Flt3 ligand stimulated proliferation of hematopoietic progenitor cells isolated from mouse fetal liver or adult mouse bone marrow. Hannum et al. (1994) concluded that FL enhances the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells. Assays of C-terminally truncated FL proteins confirmed that the N-terminal half conferred FL activity. Depletion of the Pi3k -Mtor negative regulator Pten facilitated Flt3l-driven DC development in culture.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For FLT3LG (Source: Uniprot.Org, NCBI)

Uniprot ID

P49771

Gene ID

2323

Gene Name

FLT3LG

Full Name

Fms-related tyrosine kinase 3 ligand

Weight

26.416kDa

Alternative Names

FL; FLG3L; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine FLT3LG FL, FLG3L, FLT3L fms related receptor tyrosine kinase 3 ligand fms-related tyrosine kinase 3 ligand|flt3 ligand|fms related tyrosine kinase 3 ligand

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FLT3LG, check out the FLT3LG Infographic

FLT3LG infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FLT3LG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for PA1914

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7 Customer Q&As for Anti-Flt3 ligand/FLT3LG Antibody

Question

Is this PA1914 anti-Flt3 ligand/FLT3LG antibody reactive to the isotypes of FLT3LG?

M. Thomas

Verified customer

Asked: 2020-05-08

Answer

The immunogen of PA1914 anti-Flt3 ligand/FLT3LG antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Flt-3ligand(206-220aa HWQRTRRRTPRPGEQ). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-05-08

Question

See below the WB image, lot number and protocol we used for leukocyte using anti-Flt3 ligand/FLT3LG antibody PA1914. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-10

Question

I am interested in to test anti-Flt3 ligand/FLT3LG antibody PA1914 on human leukocyte for research purposes, then I may be interested in using anti-Flt3 ligand/FLT3LG antibody PA1914 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

The products we sell, including anti-Flt3 ligand/FLT3LG antibody PA1914, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-16

Question

My question regarding product PA1914, anti-Flt3 ligand/FLT3LG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-27

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1914 anti-Flt3 ligand/FLT3LG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-27

Question

Would anti-Flt3 ligand/FLT3LG antibody PA1914 work for WB with leukocyte?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

According to the expression profile of leukocyte, FLT3LG is highly expressed in leukocyte. So, it is likely that anti-Flt3 ligand/FLT3LG antibody PA1914 will work for WB with leukocyte.

Boster Scientific Support

Answered: 2019-07-25

Question

I see that the anti-Flt3 ligand/FLT3LG antibody PA1914 works with WB, what is the protocol used to produce the result images on the product page?

M. Parker

Verified customer

Asked: 2017-06-20

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-06-20

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-Flt3 ligand/FLT3LG antibody PA1914. Let me know if you need anything else.

Z. Edwards

Verified customer

Asked: 2014-06-10

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-06-10

Size

Total: $315

SKU:PA1914

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PA1914
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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