Rat GM-CSF ELISA Kit PicoKine™

Rat GM-CSF ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Csf2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0366
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Rat GM-CSF ELISA Kit PicoKine™

See all GM-CSF products

SKU/Catalog Number

EK0366

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat GM-CSF ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Csf2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat GM-CSF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0366)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A1-K127

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0366 is reactive to Csf2 in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0366 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0366-CAP Anti-Rat Csf2 Pre-coated 96-well strip microplate 1
EK0366-ST Rat Csf2 Standard 2 vials, 10 ng/tube
EK0366-DA Rat Csf2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0300.1380.1380.2290.4230.8061.3912.271

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat GM-CSF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3021642928196414
Standard deviation1.3511.4433.891.5912.3439.33
CV (%)4.5%5.3%7.9%5.7%6.3%9.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of GM-CSF in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 130333429312.066.6%
Sample 221622724323923110.594.5%
Sample 342938139040440118.124.5%
*number of samples for each test n=16.

Gene/Protein Information For Csf2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P48750

Gene ID

116630

Gene Name

Csf2

Full Name

Granulocyte-macrophage colony-stimulating factor

Weight

16.562kDa

Superfamily

GM-CSF family

Alternative Names

colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostim; molgramostin; Sargramostim Csf2|Gm-csf, Gmcsf|colony stimulating factor 2|granulocyte-macrophage colony-stimulating factor|CSF|colony stimulating factor 2 (granulocyte-macrophage)|colony-stimulating factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Csf2, check out the Csf2 Infographic

Csf2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Csf2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0366 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Protective effects of ginsenoside Rg1 on splenocytes and thymocytes in an aging rat model induced by d-galactose

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6 Customer Q&As for Rat GM-CSF ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

H. Babu

Verified customer

Asked: 2019-06-05

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-06-05

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-06-02

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-06-02

Question

Q: if the enzyme conjugated GM-CSF antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the GM-CSF antigen?

Verified Customer

Verified customer

Asked: 2018-08-27

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-08-27

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis GM-CSF using the EK0366 Rat GM-CSF Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-06-27

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2018-06-27

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

R. Kulkarni

Verified customer

Asked: 2017-08-05

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-08-05

Question

Q: can I use citrate plasma as samples in Rat GM-CSF Picokine® ELISA Kit (Catalog # EK0366)?

Verified Customer

Verified customer

Asked: 2016-04-12

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of GM-CSF causing degradation of its protein structure. GM-CSF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the GM-CSF ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-04-12

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