Rat IGFBP5 ELISA Kit PicoKine™

Rat IGFBP5 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Igfbp5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1298
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Rat IGFBP5 ELISA Kit PicoKine™

See all IGFBP-5 products

SKU/Catalog Number

EK1298

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat IGFBP5 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Igfbp5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat IGFBP5 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1298)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-E271

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1298 is reactive to Igfbp5 in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1298 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1298-CAP Anti-Rat Igfbp5 Pre-coated 96-well strip microplate 1
EK1298-ST Rat Igfbp5 Standard 2 vials, 10 ng/tube
EK1298-DA Rat Igfbp5 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.1170.1770.2260.2790.4400.6711.0931.935

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IGFBP-5, Dilution ratio of 1:100, concentration in serum is around 180ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat IGFBP5 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3151184579332811345772
Standard deviation24.2573.4341.7826.2485.05369.4
CV (%)7.7%6.2%5.9%8%7.5%6.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IGFBP-5 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 13153053012913038.62.8%
Sample 21184111210041119110464.585.8%
Sample 357935090497449435200346.726.6%
*number of samples for each test n=16.

Gene/Protein Information For Igfbp5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P24594

Gene ID

25285

Gene Name

Igfbp5

Full Name

Insulin-like growth factor-binding protein 5

Weight

30.298kDa

Alternative Names

IBP5IBP-5; IGF-binding protein 5; IGFBP5; IGFBP-5; insulin-like growth factor binding protein 5; insulin-like growth factor-binding protein 5 Igfbp5|IGF-BP5|insulin-like growth factor binding protein 5|insulin-like growth factor-binding protein 5|IBP-5|IGF-binding protein 5|IGFBP-5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Igfbp5, check out the Igfbp5 Infographic

Igfbp5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Igfbp5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1298

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8 Customer Q&As for Rat IGFBP5 ELISA Kit PicoKine™

Question

Q: can I use citrate plasma as samples in Rat IGFBP-5 Picokine® ELISA Kit (Catalog # EK1298)?

Verified Customer

Verified customer

Asked: 2020-11-20

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IGFBP-5 causing degradation of its protein structure. IGFBP-5 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IGFBP-5 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-11-20

Question

Q: how can I thaw whole blood sample for IGFBP-5 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-10-06

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IGFBP-5 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-10-06

Question

Q: if the enzyme conjugated IGFBP-5 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IGFBP-5 antigen?

Verified Customer

Verified customer

Asked: 2020-05-11

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-05-11

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification IGFBP-5 using the EK1298 Rat IGFBP-5 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-09-30

Question

Does EK1298 measure the free or bound IGFBP-5

Verified Customer

Verified customer

Asked: 2019-01-30

Answer

EK1298 measures both the free and bound IGFBP-5.

Boster Scientific Support

Answered: 2019-01-30

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-09-22

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-09-22

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-03-29

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-03-29

Question

Q: Can IGFBP-5 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2017-09-11

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-09-11

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