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Product Info Summary
| SKU: | EK2294 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma(heparin, EDTA) |
Product info
Product Name
Rat IL-17A ELISA Kit PicoKine®
SKU/Catalog Number
EK2294
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat IL-17A ELISA Kit PicoKine® (96 Tests). Quantitate Rat IL-17A in cell culture supernatants, serum, plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat IL-17A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2294)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Ala26-Ser158
Capture/Detection Antibodies
polyclonal antibody from goat|polyclonal antibody from goat
Sensitivity
<10 pg/mL
Assay Range
156-10,000 pg/mL
Standard Dilution Instructions
See datasheet of EK2294 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2294 is reactive to Il17a in Rat samples
Validated Sample Types
cell culture supernatants, serum, plasma(heparin, EDTA)
Application Guarantee
EK2294 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Il17a
Interleukin-17A is a protein that is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2294-CAP | Anti-Rat Il17a Pre-coated 96-well strip microplate | 1 |
| EK2294-ST | Rat Il17a Standard | 2 vials, 10 ng/tube |
| EK2294-DA | Rat Il17a Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.071 | 0.127 | 0.177 | 0.305 | 0.513 | 0.878 | 1.444 | 2.210 |
Data Example Images
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Rat IL-17A PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat IL-17A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 249 | 971 | 4370 | 283 | 684 | 3639 |
| Standard deviation | 11.35 | 77.39 | 107.50 | 7.92 | 28.59 | 156.84 |
| CV (%) | 4.6% | 8.0% | 2.5% | 2.8% | 4.2% | 4.3% |
Specific Publications For Rat IL-17A ELISA Kit PicoKine® (EK2294)
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