Rat IL-6/Interleukin-6 ELISA Kit PicoKine™

Rat IL-6/Interleukin-6 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il6 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 5pg/ml. Cited in 194 publication(s).

Product Info Summary

SKU: EK0412
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Rat IL-6/Interleukin-6 ELISA Kit PicoKine™

See all IL-6 products

SKU/Catalog Number

EK0412

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat IL-6/Interleukin-6 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il6 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat IL-6/Interleukin-6 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0412)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F25-T211

Sensitivity

<5 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0412 is reactive to Il6 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0412 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0412-CAP Anti-Rat Il6 Pre-coated 96-well strip microplate 1
EK0412-ST Rat Il6 Standard 2 vials, 10 ng/tube
EK0412-DA Rat Il6 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.1830.1900.2640.3520.5300.8111.1401.820

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-6, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IL-6 in samples: 23326132 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat IL-6/Interleukin-6 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)19357915312105641466
Standard deviation9.6535.8990.3211.5540.04109.95
CV (%)5%6.2%5.9%5.5%7.1%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-6 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11931921751771848.284.5%
Sample 257963161057759922.523.7%
Sample 31531147013401471145369.764.8%
*number of samples for each test n=16.

Gene/Protein Information For Il6 (Source: Uniprot.Org, NCBI)

Uniprot ID

P20607

Gene ID

24498

Gene Name

Il6

Full Name

Interleukin-6

Weight

24.357kDa

Superfamily

IL-6 superfamily

Alternative Names

B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A Il6|ILg6, Ifnb2|interleukin 6|interleukin-6|IL-6|Interleukin 6 (interferon, beta 2)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il6, check out the Il6 Infographic

Il6 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0412 has been cited in 194 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Multifunctional Supramolecular Hydrogel for Prevention of Epidural Adhesion after Laminectomy
Species: Rat

Chen Q,Lei YQ,Liu JF,Wang ZC,Cao H.Beneficial effects of chlorogenic acid treatment on neuroinflammation after deep hypothermic circulatory arrest may be mediated through CYLD/NF-κB signaling.Brain Res.2021 Jun 30:147572.doi:10.1016/j.brainres.2021.147572.Epub ahead of print.PMID:34216581.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Yan Jiang,Li Zhao,Qing Chen,Lihong Zhou,"Exploring the Mechanism of Berberine Intervention in Ulcerative Colitis from the Perspective of Inflammation and Immunity Based on Systemic Pharmacology",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 9970240,11 pages,2021.https://doi.org/ 10.1155/2021/9970240
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Li Q,Jin Y,Ye X,Wang W,Deng G,Zhang X.Bone Marrow Mesenchymal Stem Cell-Derived Exosomal MicroRNA-133a Restrains Myocardial Fibrosis and Epithelial-Mesenchymal Transition in Viral Myocarditis Rats Through Suppressing MAML1.Nanoscale Res Lett.2021 Jul 2;16(1):111.doi:10.1186/s11671-021-03559-2.PMID:34215939.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Wang Y,Chen S,Tan J,Gao Y,Yan H,Liu Y,Yi S,Xiao Z,Wu H.Tryptophan in the diet ameliorates motor deficits in a rotenone-induced rat Parkinson‘s disease model via activating the aromatic hydrocarbon receptor pathway.Brain Behav.2021 Jun 9.doi:10.1002/brb3.2226.Epub ahead of print.PMID:34105899.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Lipopolysaccharides,I.C.I.B.B.,Fesal,M.,Seif,F.,Abdella,A.,& Amin,H.M.Middle East Journal of Applied Sciences Volume:11| Issue: 01|Jan.-March| 2021.
Species: Rat
EK0412 usage in article: APP: ELISA, SAMPLE:LIVER TISSUE, DILUTION:NA

Ming Tang,Xia Gao,Ting Geng,Xialin Chen,Jiajia Wang,Canjie Shen,Huifang Gao,Mengyu Qian,Zhenzhong Wang,Liang Cao,Wei Xiao,Metabolomics analysis of the therapeutic effects of Qiwei Tongbi oral liquid on rheumatoid arthritis in rats,Journal of Pharmaceutical and Biomedical Analysis, Volume 202,2021,114166,ISSN 0731-7085,https://doi.org/10.1016/j.jpba.2021.114166.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Effect of α-Tocopherol on Cytokines Production, Oxidative Stress, and Liver Injury Induced by Lipopolysaccharides in Male Albino Rats. Amin,H. M.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Cheng Wang,Liang Xian,Shaorui Zheng et al.Cranial Venous Outflow Obstruction Promotes Neuroinflammation And Edema Via ADAM17/solTNF-α/NF-κB Pathway In A Rat Subdural Hematoma Model,11 May 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-487715/v1]
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION: NA

Motavallian A,Bouzari S,Zamani E,Karimian P,Dabirian S,Molavi M,Torshkooh FA.An investigation of the anti-inflammatory effects of gabapentin on acetic acid-induced colitis in rats.Mol Biol Rep.2021 Apr 29.doi:10.1007/s11033-021-06357-2.Epub ahead of print.PMID:33928442.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA

Xu H,Wang Y,Luo Y.OTULIN is a new target of EA treatment in the alleviation of brain injury and glial cell activation via suppression of the NF-κB signalling pathway in acute ischaemic stroke rats.Mol Med.2021 Apr 9;27(1):37.doi:10.1186/s10020-021-00297-0.PMID:33836646;PMCID:PMC8035756.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Lixin Xia,Shuo Wang,Zhiwen Jiang,Jinhua Chi,Shuqin Yu,Hongjian Li,Yijie Zhang,Lihua Li,Changren Zhou,Wanshun Liu,Baoqin Han,Hemostatic performance of chitosan-based hydrogel and its study on biodistribution and biodegradability in rats,Carbohydrate Polymers,2021,117965,ISSN 0144-8617,https://doi.org/10. 1016/j.carbpol.2021.117965.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE: SERUM, DILUTION:NA

Abukhalil MH, Althunibat OY, Aladaileh SH, Al-Amarat W, Obeidat HM, Al-Khawalde AAA, Hussein OE, Alfwuaires MA, Algefare AI, Alanazi KM, Al-Swailmi FK, Arab HH, Mahmoud AM. Galangin attenuates diabetic cardiomyopathy through modulating oxidative stress, inflammation and apoptosis in rats. Biomed Pharmacother. 2021 Mar 19:111410. doi: 10.1016/j.biopha.2021.111410. Epub ahead of print. PMID: 33752930.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:HEART TISSUE, DILUTION:NA

Zhang P,Gan Z,Tang L,Zhou L,Huang X,Wang J.Exosomes from microRNA-145-5p-modified HUCB-MSCs attenuate CCl4-induced hepatic fibrosis via down-regulating FSCN1 expression.Life Sci.2021 Mar 29:119404.doi:10.1016/j.lfs.2021.119404.Epub ahead of print.PMID:33794251.
Species: Human,Rat

Yin Q,Du T,Yang C,Li X,Zhao Z,Liu R,Yang B,Liu B.Gadd45b is a novel mediator of depression-like behaviors and neuroinflammation after cerebral ischemia.Biochem Biophys Res Commun.2021 Mar 27;554:107-113.doi:10.1016/j.bbrc.2021.03.104.Epub ahead of print.PMID:33784505.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUS TISSUE, DILUTION:NA

Ouyang, S.,Chen, W.,Gaofeng, Z.,Changcheng, L.,Guoping, T.,Minyan, Z. ... Luo, J. (2021). Cyanidin‑3‑O‑β‑glucoside protects against pulmonary artery hypertension induced by monocrotaline via the TGF‑β1/p38 MAPK/CREB signaling pathway. Molecular Medicine Reports,23, 338.https://doi.org/10.3892/mmr.2021.11977
Species: Human,Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Xiaoling Zhou,Xingwei Xiang,Yufang Zhou,Tianyi Zhou,Shanggui Deng,Bin Zheng,Pingan Zheng,Protective effects of Antarctic krill oil in dextran sulfate sodium-induced ulcerative colitis mice,Journal of Functional Foods,Volume 79,2021,104394,ISSN 1756-4646,h
Species: Mouse
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Martins Ekor,Phyllis E. Owusu Agyei,Ernest Obese,Robert P. Biney,Isaac T. Henneh,Meshack Antwi-Adjei,Ewura S. Yahaya,Gordon Amoakohene,Patrick K. Akakpo bioRxiv 2021.02.15.431245;doi:https://doi.org/10.1101/2021.02.15.431245
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

He C,Su C,Zhang W,Zhou Q,Shen X,Yang J,Shi N.Modulatory Potential of LncRNA Zfas1 for Inflammation and Neuronal Apoptosis in Temporal Lobe Epilepsy.Yonsei Med J.2021 Mar;62(3):215-223.doi:10.3349/ymj.2021.62.3.215.PMID:33635011.
Species: Human,Rat
EK0412 usage in article: APP:ELISA, SAMPLE:NEURON, DILUTION:NA

Jia Q,Zhang H,Su Y,Liu X,Bai J,Lang W,Shi Q,Feng M.Strictosamide alleviates the inflammation in an acute ulcerative colitis (UC) model.J Physiol Biochem.2021 Feb 17.doi:10.1007/s13105-021-00796-y.Epub ahead of print.PMID:33595775.
Species: Human,Mouse,Rat
EK0412 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA

Hongbei Xu,You Wang,Yong Luo et al. OTULIN is a New Target for EA Treatment in Alleviating Brain Injury and The Activation of Glial Cells By Depressing NF-κB Signalling Pathway in Acute Ischaemic Stroke Rats,28 January 2021,PREPRINT (Version 1) available
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

The maternal western diet exacerbates the inflammatory response in the offspring, but does not alter the action of the non-steroidal antiinflammatory drug. Nathalia Caroline de Oliveira Melo,Nathália Cavalcanti de Morais Araújo,Alexandra Rabello Freire,Maria Bernadete de Souza Maia,Elizabeth do Nascimento
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SKIN TISSUE, DILUTION:NA

Li J,Xie Y,Zhao P,Qin Y,Oliver BG,Tian Y,Li S,Wang M,Liu X. A chinese herbal formula ameliorates COPD by inhibiting the inflammatory response via downregulation of p65, JNK, and p38. Phytomedicine. 2021 Jan 20;83:153475.doi:10.1016/j.phymed.2021.153475.Ep
Species: Human,Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM AND BALF, DILUTION:NA

Haiyang Zhang,Wenjing Jiao,Hailin Cui,Qinghong Sun,Honggang Fan,Combined exposure of alumina nanoparticles and chronic stress exacerbates hippocampal neuronal ferroptosis via activating IFN-γ/ASK1/JNK signaling pathway in rats,Journal of Hazardous Materia
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUS, DILUTION:NA

Manman Zhao,Junling Gao,Changmeng Cui,Yanan Zhang,Xiaohua Jiang,Jianzhong Cui, "Inhibition of PTEN Ameliorates Secondary Hippocampal Injury and Cognitive Deficits after Intracerebral Hemorrhage: Involvement of AKT/FoxO3a/ATG-Mediated Autophagy",Oxidative
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:NA

Chen T,Liu WB,Qian X,Xie KL,Wang YH.The AMPAR antagonist perampanel protects the neurovascular unit against traumatic injury via regulating Sirt3.CNS Neurosci Ther.2021 Jan 9.doi:10.1111/cns.13580.Epub ahead of print.PMID:33421349.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Liu Yuan,Luo Pei,Zhang Ji-Zhong,Chen Xiao-Yi,Li Li-Min,Wu You-Jiao,Yin Qiao-Zhi,Huang Li.Identification of Active Compounds From Yi Nationality Herbal Formula Wosi Influencing COX-2 and VCAM-1 Signaling. Frontiers in Pharmacology,11.2020.1690.https://www.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Jiang,Guoyong and Lei,AItong and Chen,Yi and Yu,Qiang and Xie,Jianhua and Yang,Ying and Yuan,Tongji and Su, Dan", "Protective effects of Ganoderma atrum polysaccharide against acrylamide-induced inflam mation and oxidative damage in rats","Food Funct.","2
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Yingchao Kang, Yaping Sun, Tiantian Li, Zelin Ren,Garcinol protects against cerebral ischemia-reperfusion injury in vivo and in vitro by inhibiting inflammation and oxidative stress, Molecular and Cellular Probes ,2020, 101672,ISSN 0890-8508,https://doi.o
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE AND PC12 CELLS, DILUTION:NA

Wang,Q.,Liu,S.,& Han,Z.(2020).miR-339-3p regulated acute pancreatitis induced by caerulein through targeting TNF receptor-associated factor 3 in AR42J cells, Open Life Sciences , 15(1), 912-922. doi:https://doi.org/10.1515/biol-2020-0084.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:MACROPHAGE, DILUTION:NA

Zhang K,Liu R,Gao Y,Ma W,Shen W.Electroacupuncture Relieves LPS-Induced Depression-Like Behaviour in Rats Through IDO-Mediated Tryptophan-Degrading Pathway.Neuropsychiatr Dis Treat.2020 Oct 5;16:2257-2266.doi:10.2147/NDT.S274778.PMID:33116524;PMCID:PMC754
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUS AND SERUM, DILUTION:NA

Li J,Ma J,Tian Y,Zhao P,Liu X,Dong H,Zheng W,Feng S,Zhang L,Wu M,Zhu L,Liu S,Zhao D.Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K/mTOR signa
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM AND BALF, DILUTION:NA

Wang Y,Zhang L,Li L,Hu H,Pan P,Zhang B,Ning W,Zhao M,Wang S.Electroacupuncture Improves Blood Pressure in SHRs by Regulating the Immune Balance between Th17 and Treg.Evid Based Complement Alternat Med.2020 Jul 7;2020:5375981.doi:10.1155/2020/5375981.PMID:
Species: Rat

Liu Y,Zhang X,Yan D,Wang Y,Wang N,Liu Y,Tan A,Chen X,Yan H.Chronic acrylamide exposure induced glia cell activation, NLRP3 infl-ammasome upregulation and cognitive impairment.Toxicol Appl Pharmacol.2020 Apr 15;393:114949.doi:10.1016/j.taap.2020.114949.Epu
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Liu L,Zhou J,Wang Y,Qi T,Wang Z,Chen L,Suo N.Imatinib inhibits oxidative stress response in spinal cord injury rats by activating Nrf2/HO-1 signaling pathway.Exp Ther Med.2020 Jan;19(1):597-602.doi:10.3892/etm.2019.8270.Epub 2019 Dec 2.PMID:31897102;PMCID
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:SPINAL CORD TISSUE, DILUTION:NA

Feriani A,Tir M,Arafah M,Gómez-Caravaca AM,Contreras MDM,Nahdi S,Taamalli A,Allagui MS,Alwasel S,Segura-Carretero A,Harrath AH,Tlili N. Schinus terebinthifolius fruits intake ameliorates metabolic disorders, inflammation, oxidative stress, and related vas
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA

Mao H,Huang Q,Liu Y. MEG3 aggravates hypoxia/reoxygenation induced apoptosis of renal tubular epithelial cells via the miR-129-5p/HMGB1 axis. J Biochem Mol Toxicol.2020 Nov 11:e22649.doi:10.1002 /jbt .22 649. Epub ahead of print.PMID:33175458.
Species: Human,Rat

Liu S, Wang Y, Lu S, Hu J, Zeng X, Liu W, Wang Y, Wang Z. Sacubitril/valsartan treatment relieved the progression of established pulmonary hypertension in rat model and its mechanism. Life Sci. 2020 Dec 10:118877. doi: 10.1016/j.lfs.2020.118877. Epub ahea
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA

Zheng X,Cai X,Ye F,Li Y,Wang Q,Zuo Z,Huang W,Wang Z. Perioperative Dexmedetomidine attenuates brain ischemia reperfusion injury possibly via up-regulation of astrocyte Connexin 43. BMC Anesthesiol. 2020 Dec 7;20(1):299.doi: 10.1186/s12871-020-01211-7.PMID
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Wang Q, Xu GX, Tai QH, Wang Y. Lipoxin A4 Reduces Ventilator-Induced Lung Injury in Rats with Large-Volume Mechanical Ventilation. Mediators Inflamm. 2020 Nov 22;2020:6705985. doi: 10.1155/2020/6705985. PMID: 33299377; PMCID: PMC7704204.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA

Cao Y, Wang L, Lin LT, Wang XR, Ma SM, Yang NN, Fan H, Fisher M, Yang JW. Acupuncture attenuates cognitive deficits through α7nAChR mediated anti-inflammatory pathway in chronic cerebral hypoperfusion rats. Life Sci. 2020 Nov 5:118732. doi: 10.1016/j.lfs.
Species: Rat
EK0412 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUS, DILUTION:NA

Zhang, B., Guo, Z., Lai, S., & Chen, H. (2020). Interference with miR-210 Alleviated Renal Injury in Septic Rats by Inhibiting JAK-STAT Pathway. Inflammation, 1-10.

Toxicology study for magnetic injection of prednisolone into the rat cochlea.

The effects of Jiao-Tai-Wan on sleep, inflammation and insulin resistance in obesity-resistant rats with chronic partial sleep deprivation

Pachymic acid ameliorates sepsis-induced acute kidney injury by suppressing inflammation and activating the Nrf2/HO-1 pathway in rats

The Effects of Resveratrol on Inflammation and Oxidative Stress in a Rat Model of Chronic Obstructive Pulmonary Disease

Novel therapeutic approach by nicotine in experimental model of multiple sclerosis

Sialic acid-modified solid lipid nanoparticles as vascular endothelium-targeting carriers for ischemia-reperfusion-induced acute renal injury

Levels of serum pentraxin 3, IL-6, fetuin A and insulin in patients with rheumatoid arthritis

Changes in mediators of inflammation and pro-thrombosis after 12 months of dietary modification in adults with metabolic syndrome

Effect of fermented camel milk on glucose metabolism, insulin resistance, and inflammatory biomarkers of adolescents with metabolic syndrome: A double-blind, randomized, crossover trial

Dendrobium Officinale Kimura et Migo Ameliorates Insulin Resistance in Rats with Diabetic Nephropathy

Valproic acid attenuates traumatic spinal cord injury-induced inflammation via STAT1 and NF-?B pathway dependent of HDAC3

THE IMPACT OF THE USE OF SYMBIOTICS IN THE PROGRESSION OF NONALCOHOLIC FATTY LIVER DISEASE IN A RAT MODEL

Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by ?-ray irradiation in mice

Potassium 2-(1-hydroxypentyl)-benzoate attenuates neuronal apoptosis in neuron?astrocyte co-culture system through neurotrophy and neuroinflammation pathway

Resveratrol protects against early polymicrobial sepsis-induced acute kidney injury through inhibiting endoplasmic reticulum stress-activated NF-?B pathway

Protective Effects of Cucurbitacin B on Acute Lung Injury Induced by Sepsis in Rats

Protective effect of geraniol inhibits inflammatory response, oxidative stress and apoptosis in traumatic injury of the spinal cord through modulation of NF-?B and p38 MAPK

Investigation of the Effects and Mechanisms of Mai Tong Formula on Lower Limb Macroangiopathy in a Spontaneous Diabetic Rat Model

Changes in the Interstitial Cells of Cajal and Immunity in Chronic Psychological Stress Rats and Therapeutic Effects of Acupuncture at the Zusanli Point (ST36)

Sequential Treatments with Tongsai and Bufei Yishen Granules Reduce Inflammation and Improve Pulmonary Function in Acute Exacerbation-Risk Window of Chronic Obstructive Pulmonary Disease in Rats

Anti-Diabetic Effect of Portulaca oleracea L Polysaccharideandits Mechanism in Diabetic Rats

Protective effect of Astragaloside IV against sepsis-induced acute lung injury in rats

Expression of scavenger receptor A in rat's liver tissue during acute obstructive cholangitis and its significance

Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells

Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway

GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-?B Pathway

Extracts of Bauhinia championii (Benth) Benth attenuate the in?ammatory response in a rat model of collagen-induced arthritis

Laboratory environment and bio-medical experience: the impact of administration technique on the quality of immune-behavior data results in stress experience

Wound healing treatment by high frequency ultrasound, microcurrent, and combined therapy modifies the immune response in rats

Decrease of CD68 Synovial Macrophages in Celastrol Treated Arthritic Rats

Propofol pretreatment attenuates lipopolysaccharide-induced acute lung injury in rats by activating the phosphoinositide-3-kinase/Akt pathway

TNF?-Mediated Loss of ?-Catenin/E-Cadherin Association and Subsequent Increase in Cell Migration Is Partially Restored by NKX3.1 Expression in Prostate Cells

Epinephrine Enhances the Response of Macrophages under LPS Stimulation

Treatment of acute lung injury by targeting MG53-mediated cell membrane repair

Inhibition of ROS-Activated p38MAPK Pathway is Involved in the Protective Effect of H2S Against Chemical Hypoxia-Induced Inflammation in PC12 Cells

Comparison of Anti-Inflammatory and Anti-Nociceptive Activities of Curcuma Wenyujin Y.H. Chen et C. Ling and Scutellaria Baicalensis Georgi

Whether Co-Administration of Garlic has Negative Influence on Scutellaria Baicalensis Georgi in Treating Models Rats with Pelvic Inflammation?

Yang S, Zhang J, Wang S, Zhao X, Shi J. Oncotarget. 2017 Aug 24;8(53):91185-91198. doi: 10.18632/oncotarget.20434. eCollection 2017 Oct 31. SOCS2 overexpression alleviates diabetic nephropathy in rats by inhibiting the TLR4/NF-κB pathway

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10 Customer Q&As for Rat IL-6/Interleukin-6 ELISA Kit PicoKine™

Question

Q: which procedure should I follow in order to thaw whole blood sample for IL-6 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-05-10

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-6 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-05-10

Question

I would like to know more about Rat IL-6 ELISA Kit antibody. Could this be used also as neutralizing antibody? As it stated in the product notes that this antibody can be used to block IL-6/IL-6R interactions. You think it is just the same as neutralizing antibody?

Verified Customer

Verified customer

Asked: 2020-03-19

Answer

The Rat IL-6 ELISA Kit antibody can be used to prevent IL-6 binding to its receptor, therefore it could be used in a neutralizing type of assay.

Boster Scientific Support

Answered: 2020-03-19

Question

To Technical Support: I would like to inquire if this Rat IL-6 ELISA Kit can be used for urine samples?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

The Rat IL-6 ELISA Kit has been successfully tested with urine samples, we can guarantee that it will worked well. However, we would recommend to start a minimal dilution (such as 2-fold) since based on our experience cytokine levels are often very low in urine. Here

Boster Scientific Support

Answered: 2020-01-08

Question

Q: how to analyze ELISA data? I measured IL-6 level in plasma.

W. Carter

Verified customer

Asked: 2019-11-09

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-11-09

Question

Is the Rat IL-6 PicoKine

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

We haven't tested

Boster Scientific Support

Answered: 2019-06-04

Question

Q: can I use citrate plasma as samples in Rat IL-6 Picokine® ELISA Kit (Catalog # EK0412)?

Verified Customer

Verified customer

Asked: 2019-03-18

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-6 causing degradation of its protein structure. IL-6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-6 ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-03-18

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-02-25

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-02-25

Question

Can EK0412 be used with cytosolic fractions?

Verified customer

Asked: 2019-02-08

Answer

We haven't tested tissue lysates/cytosolic fractions with Rat IL-6 ELISA Kit PicoKine EK0412. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend to run a pilot test to confirm the effect of endogeous biotin first. Add tissue homogenates into the wells then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.

Boster Scientific Support

Answered: 2019-02-08

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-11-16

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-11-16

Question

Q: What is the optimal O.D. value for IL-6 ELISA kit? I performed your IL-6 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-6 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2015-04-07

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-6 ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of IL-6 that can be declared/interpreted as positive by the above standard is 5pg/ml.

Boster Scientific Support

Answered: 2015-04-07

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