Rat Lipocalin-2/NGAL ELISA Kit PicoKine™

Rat Lipocalin-2/NGAL ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Lcn2 in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml. Cited in 14 publication(s).

Product Info Summary

SKU: EK0855
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin) and urine.

Product Name

Rat Lipocalin-2/NGAL ELISA Kit PicoKine™

See all Lipocalin-2/NGAL products

SKU/Catalog Number

EK0855

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat Lipocalin-2/NGAL ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Lcn2 in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat Lipocalin-2/NGAL ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0855)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-N198

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0855 is reactive to Lcn2 in Rat samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin) and urine.

Application Guarantee

EK0855 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0855-CAP Anti-Rat Lcn2 Pre-coated 96-well strip microplate 1
EK0855-ST Rat Lcn2 Standard 2 vials, 10 ng/tube
EK0855-DA Rat Lcn2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156312625125025005000
O.D.0.0670.1350.1810.3120.4840.7801.2491.737

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat Lipocalin-2/NGAL ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12175031881267273167
Standard deviation6.0550.25168.967.356.7177.35
CV (%)5%6.7%5.3%5.8%7.8%5.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Lipocalin-2/NGAL in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11211181021171147.366.4%
Sample 275075169374073323.773.2%
Sample 331882684284731442965208.957%
*number of samples for each test n=16.

Gene/Protein Information For Lcn2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P30152

Gene ID

170496

Gene Name

Lcn2

Full Name

Neutrophil gelatinase-associated lipocalin

Weight

22.476kDa

Superfamily

calycin superfamily

Alternative Names

24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin Lcn2|Sip24|lipocalin 2|neutrophil gelatinase-associated lipocalin|NGAL|alpha-2-microglobulin-related protein|alpha-2U globulin-related protein|lipocalin 2 (oncogene 24p3)|lipocalin 24p3|p25|siderocalin LCN2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Lcn2, check out the Lcn2 Infographic

Lcn2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Lcn2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0855 has been cited in 14 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Guo S,Guo L,Fang Q,Yu M,Zhang L,You C,Wang X,Liu Y,Han C.Astaxanthin protects against early acute kidney injury in severely burned rats by inactivating the TLR4/MyD88/NF-κB axis and upregulating heme oxygenase-1.Sci Rep.2021 Mar 23;11 (1):6679.doi:10.1038/s41598-021-86146-w.PMID:33758309;PMCID:PMC7988001.
Species: Rat
EK0855 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Songxue Guo,Linsen Guo,Quan Fang et al.Astaxanthin Protects Against Early Acute Kidney Injury in Severely-Burned Rats Through Inactivating TLR4/MyD88/NF-kB Axis and Upregulating Heme Oxygenase-1, 07 October 2020, PREPRINT (Version 1) available at Res
Species: Rat
EK0855 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA

The renoprotective effects of mannitol and udenafil in renal ischemia-reperfusion injury model

The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats

Renal Ischemia/Reperfusion Injury in Diabetic Rats: The Role of Local Ischemic Preconditioning

Esculin improves dyslipidemia, inflammation and renal damage in streptozotocin-induced diabetic rats

The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model

Erkılıç E, Kesimci E, Alaybeyoğlu F, Kılınç I, Tural R, Yazgan A, Gümüş T, Sepici Dinçel A, Dumlu EG, Kanbak O. Drug Des Devel Ther. 2017 Mar 8;11:677-683. doi: 10.2147/DDDT.S126701. eCollection 2017. Does remifentanil attenuate renal ischemia-rep...

Guo Sx, Zhou Hl, Huang Cl, You Cg, Fang Q, Wu P, Wang Xg, Han Cm. Mar Drugs. 2015 Apr 13;13(4):2105-23. Doi: 10.3390/Md13042105. Astaxanthin Attenuates Early Acute Kidney Injury Following Severe Burns In Rats By Ameliorating Oxidative Stress And M...

Guo Sx, Fang Q, You Cg, Jin Yy, Wang Xg, Hu Xl, Han Cm. J Transl Med. 2015 Jun 6;13:183. Doi: 10.1186/S12967-015-0548-3. Effects Of Hydrogen-Rich Saline On Early Acute Kidney Injury In Severely Burned Rats By Suppressing Oxidative Stress Induced A...

Koca U, Olguner ??g, Erg??r Bu, Altekin E, Ta??d????en A, Duru S, Girgin P, G??nd??z K, Cilaker M??c??l?? S, G??zelda?? S, Akku?? M. Scientificworldjournal. 2013;2013:292687. Doi: 10.1155/2013/292687. Epub 2013 Feb 13. The Effects Of Dexmedetomidi...

Olguner Cg, Koca U, Altekin E, Erg??r Bu, Duru S, Girgin P, Ta??d????en A, G??nd??z K, G??zelda?? S, Akku?? M, Micili Sc. Exp Ther Med. 2013 Jun;5(6):1581-1588. Epub 2013 Apr 2. Ischemic Preconditioning Attenuates Lipid Peroxidation And Apoptosis ...

Sun Q, Meng Qt, Jiang Y, Liu Hm, Lei Sq, Su Wt, Duan Wn, Wu Y, Xia Zy, Xia Zy. Plos One. 2013 Dec 4;8(12):E80859. Doi: 10.1371/Journal.Pone.0080859. Ecollection 2013. Protective Effect Of Ginsenoside Rb1 Against Intestinal Ischemia-Reperfusion Ind...

Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.

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10 Customer Q&As for Rat Lipocalin-2/NGAL ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-04-22

Question

Can EK0855 be used for stool supernatant samples?

Verified customer

Asked: 2020-01-17

Answer

Our lab hasn't worked on stool supernatant samples with the Rat Lipocalin-2/NGAL ELISA Kit PicoKine™ (EK0855). We suggest to run a pilot test. In theory, the kit is able to detect the target protein if the expression level of NGAL in the stool supernatant samples falls in the assay range of EK0855.

Boster Scientific Support

Answered: 2020-01-17

Question

Q: how do I analyze ELISA data? I measured Lipocalin-2 level in serum.

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-01-14

Question

Is there supposed to be a washstep in between step 5 and step 6 of the ELISA protocols in the datasheet?

Verified customer

Asked: 2019-04-09

Answer

There is no wash step in between step 5 and step 6 of the ELISA protocols in the datasheet.

Boster Scientific Support

Answered: 2019-04-10

Question

Q: if the enzyme conjugated Lipocalin-2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Lipocalin-2 antigen?

Verified Customer

Verified customer

Asked: 2017-07-17

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-07-17

Question

I am preparing to use your Rat Lcn2 ELISA cat#EK0855. In reading the procedure I noticed there is not a wash after step 5 and before step 6. This is after incubating samples/standard and before addition of the 1xbiotinylated Anti-Rat Lcn2 antibody. Is this correct?

Verified Customer

Verified customer

Asked: 2017-06-14

Answer

There is no wash step in between step 5 and step 6 of the ELISA protocols in the datasheet.

Boster Scientific Support

Answered: 2017-06-14

Question

Q: how to thaw whole blood sample for Lipocalin-2 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2017-06-14

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Lipocalin-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2017-06-14

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-04-12

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-04-12

Question

The customer would like to know if (#LB001-02 from Welgene) can be used as washing buffer for EK0855 kit. The composition is 200mg/L KCl, 200mg/L KH2PO4, 8000mg/L NaCl, 2160mg/L Na2HPO4

Verified Customer

Verified customer

Asked: 2014-12-26

Answer

The recipe of our wash buffer is different from that of LB002-01. We haven't used the same buffer as LB002-01. And we do not suggest the customer use it. Please use the wash buffer included in the kit. Or prepare wash buffer as the method described below. Washing buffer (neutral PBS or TBS). ? Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450?l of purified acetic acid or 700?l of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. ? Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

Boster Scientific Support

Answered: 2014-12-26

Question

Q: is it okay to use citrate plasma as samples in Rat Lipocalin-2 Picokine® ELISA Kit (Catalog # EK0855)?

K. Scott

Verified customer

Asked: 2014-05-18

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Lipocalin-2 causing disruption of its protein structure. Lipocalin-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Lipocalin-2 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2014-05-18

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