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Product Info Summary
| SKU: | EK0855 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin) and urine. |
Product info
Product Name
Rat Lipocalin-2/NGAL ELISA Kit PicoKine®
SKU/Catalog Number
EK0855
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Lipocalin-2/NGAL ELISA Kit PicoKine® (96 Tests). Quantitate Rat Lcn2 in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Lipocalin-2/NGAL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0855)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q21-N198
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0855 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0855 is reactive to Lcn2 in Rat samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin) and urine.
Application Guarantee
EK0855 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Lcn2
Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant rat NGAL, consisting of 177 amino acids with the molecular mass of 21kDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0855-CAP | Anti-Rat Lcn2 Pre-coated 96-well strip microplate | 1 |
| EK0855-ST | Rat Lcn2 Standard | 2 vials, 10 ng/tube |
| EK0855-DA | Rat Lcn2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.031 | 0.145 | 0.225 | 0.418 | 0.739 | 1.09 | 1.661 | 2.13 |
Data Example Images
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Rat Lipocalin-2/NGAL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Lcn2, Dilution ratio of 1:500, concentration in serum is around 200 ng/ml..
Some articles we found to cite concentrations of Lcn2 in samples: 24551041 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Lipocalin-2/NGAL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 121 | 750 | 3188 | 126 | 727 | 3167 |
| Standard deviation | 6.05 | 50.25 | 168.96 | 7.3 | 56.7 | 177.35 |
| CV (%) | 5% | 6.7% | 5.3% | 5.8% | 7.8% | 5.6% |
Specific Publications For Rat Lipocalin-2/NGAL ELISA Kit PicoKine® (EK0855)
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Rat Lipocalin-2/NGAL ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-04-22
Question
Can EK0855 be used for stool supernatant samples?
Verified customer
Asked: 2020-01-17
Answer
Our lab hasn't worked on stool supernatant samples with the Rat Lipocalin-2/NGAL ELISA Kit PicoKine™ (EK0855). We suggest to run a pilot test. In theory, the kit is able to detect the target protein if the expression level of NGAL in the stool supernatant samples falls in the assay range of EK0855.
Boster Scientific Support
Answered: 2020-01-17
Question
Q: how do I analyze ELISA data? I measured Lipocalin-2 level in serum.
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2020-01-14
Question
Is there supposed to be a washstep in between step 5 and step 6 of the ELISA protocols in the datasheet?
Verified customer
Asked: 2019-04-09
Answer
There is no wash step in between step 5 and step 6 of the ELISA protocols in the datasheet.
Boster Scientific Support
Answered: 2019-04-10
Question
Q: if the enzyme conjugated Lipocalin-2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Lipocalin-2 antigen?
Verified Customer
Verified customer
Asked: 2017-07-17
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-07-17
Question
I am preparing to use your Rat Lcn2 ELISA cat#EK0855. In reading the procedure I noticed there is not a wash after step 5 and before step 6. This is after incubating samples/standard and before addition of the 1xbiotinylated Anti-Rat Lcn2 antibody. Is this correct?
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
There is no wash step in between step 5 and step 6 of the ELISA protocols in the datasheet.
Boster Scientific Support
Answered: 2017-06-14
Question
Q: how to thaw whole blood sample for Lipocalin-2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Lipocalin-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-06-14
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-04-12
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-04-12
Question
The customer would like to know if (#LB001-02 from Welgene) can be used as washing buffer for EK0855 kit. The composition is 200mg/L KCl, 200mg/L KH2PO4, 8000mg/L NaCl, 2160mg/L Na2HPO4
Verified Customer
Verified customer
Asked: 2014-12-26
Answer
The recipe of our wash buffer is different from that of LB002-01. We haven't used the same buffer as LB002-01. And we do not suggest the customer use it. Please use the wash buffer included in the kit. Or prepare wash buffer as the method described below. Washing buffer (neutral PBS or TBS). ? Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450?l of purified acetic acid or 700?l of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. ? Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Boster Scientific Support
Answered: 2014-12-26
Question
Q: is it okay to use citrate plasma as samples in Rat Lipocalin-2 Picokine® ELISA Kit (Catalog # EK0855)?
K. Scott
Verified customer
Asked: 2014-05-18
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Lipocalin-2 causing disruption of its protein structure. Lipocalin-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Lipocalin-2 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2014-05-18
