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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin) and urine.|
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Rat Lipocalin-2/NGAL ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat Lipocalin-2/NGAL ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Lcn2 in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Rat Lipocalin-2/NGAL ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0855)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Q21-N198
78 pg/ml - 5,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0855 is reactive to Lcn2 in Rat samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin) and urine.
EK0855 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0855-CAP||Anti-Rat Lcn2 Pre-coated 96-well strip microplate||1|
|EK0855-ST||Rat Lcn2 Standard||2 vials, 10 ng/tube|
|EK0855-DA||Rat Lcn2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat Lipocalin-2/NGAL PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Lipocalin-2/NGAL ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Lipocalin-2/NGAL in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Lcn2 (Source: Uniprot.Org, NCBI)
Neutrophil gelatinase-associated lipocalin
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin Lcn2|Sip24|lipocalin 2|neutrophil gelatinase-associated lipocalin|NGAL|alpha-2-microglobulin-related protein|alpha-2U globulin-related protein|lipocalin 2 (oncogene 24p3)|lipocalin 24p3|p25|siderocalin LCN2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Lcn2, check out the Lcn2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Lcn2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0855 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Guo S,Guo L,Fang Q,Yu M,Zhang L,You C,Wang X,Liu Y,Han C.Astaxanthin protects against early acute kidney injury in severely burned rats by inactivating the TLR4/MyD88/NF-κB axis and upregulating heme oxygenase-1.Sci Rep.2021 Mar 23;11 (1):6679.doi:10.1038/s41598-021-86146-w.PMID:33758309;PMCID:PMC7988001.
EK0855 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Songxue Guo,Linsen Guo,Quan Fang et al.Astaxanthin Protects Against Early Acute Kidney Injury in Severely-Burned Rats Through Inactivating TLR4/MyD88/NF-kB Axis and Upregulating Heme Oxygenase-1, 07 October 2020, PREPRINT (Version 1) available at Res
EK0855 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA
The renoprotective effects of mannitol and udenafil in renal ischemia-reperfusion injury model
The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats
Renal Ischemia/Reperfusion Injury in Diabetic Rats: The Role of Local Ischemic Preconditioning
Esculin improves dyslipidemia, inflammation and renal damage in streptozotocin-induced diabetic rats
The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model
Erkılıç E, Kesimci E, Alaybeyoğlu F, Kılınç I, Tural R, Yazgan A, Gümüş T, Sepici Dinçel A, Dumlu EG, Kanbak O. Drug Des Devel Ther. 2017 Mar 8;11:677-683. doi: 10.2147/DDDT.S126701. eCollection 2017. Does remifentanil attenuate renal ischemia-rep...
Guo Sx, Zhou Hl, Huang Cl, You Cg, Fang Q, Wu P, Wang Xg, Han Cm. Mar Drugs. 2015 Apr 13;13(4):2105-23. Doi: 10.3390/Md13042105. Astaxanthin Attenuates Early Acute Kidney Injury Following Severe Burns In Rats By Ameliorating Oxidative Stress And M...
Guo Sx, Fang Q, You Cg, Jin Yy, Wang Xg, Hu Xl, Han Cm. J Transl Med. 2015 Jun 6;13:183. Doi: 10.1186/S12967-015-0548-3. Effects Of Hydrogen-Rich Saline On Early Acute Kidney Injury In Severely Burned Rats By Suppressing Oxidative Stress Induced A...
Koca U, Olguner ??g, Erg??r Bu, Altekin E, Ta??d????en A, Duru S, Girgin P, G??nd??z K, Cilaker M??c??l?? S, G??zelda?? S, Akku?? M. Scientificworldjournal. 2013;2013:292687. Doi: 10.1155/2013/292687. Epub 2013 Feb 13. The Effects Of Dexmedetomidi...
Olguner Cg, Koca U, Altekin E, Erg??r Bu, Duru S, Girgin P, Ta??d????en A, G??nd??z K, G??zelda?? S, Akku?? M, Micili Sc. Exp Ther Med. 2013 Jun;5(6):1581-1588. Epub 2013 Apr 2. Ischemic Preconditioning Attenuates Lipid Peroxidation And Apoptosis ...
Sun Q, Meng Qt, Jiang Y, Liu Hm, Lei Sq, Su Wt, Duan Wn, Wu Y, Xia Zy, Xia Zy. Plos One. 2013 Dec 4;8(12):E80859. Doi: 10.1371/Journal.Pone.0080859. Ecollection 2013. Protective Effect Of Ginsenoside Rb1 Against Intestinal Ischemia-Reperfusion Ind...
Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.
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